脱毛剂量环磷酰胺对貉皮肤组织及毛被脱换的影响

用脱毛剂量（２８ｍｇ／ｋｇ体重）的环磷酰胺。对春季换毛前的貉（Ｎｙｃｔｅｒｅｕｔｅｓ ｐｒｏｃｙｏｎｏｉｄｅｓ）进行肌注。研究其脱毛机理。在注药后的不同时间，取其背部皮肤样品少许，分别固宇下１０％的福尔马林和２％的戊二醛中。采用常规的石蜡和超薄切片法制片。对其毛皮、毛被及皮脂等影响的组织进行观察。结果表明，注药后第１天毛囊结构已发生变化；第７天针毛囊和毛球角质经；第１５天毛囊结构基本正常。毛囊上升

Impact of Molting Dose Injection of Cyclophosphamide on the Skin Tissue and Molting in Raccoon Dogs

An intramuscular injection of molting dose (28mg/kg) of cyclophosphamide was conducted before spring molting of raccoon dogs (Nyctereutes procyonoides) to explore the molting mechanism Small pieces of dorsal skin were sampled every certain interval after injection, and fixed in Formalin (10%) and Glutaric dialdehyde (2%) immediately Both wax and ultrathin sections were made with above samples for both photo-microscopic and transmission electron microscopic detection of the impact of injected cyclophosphamide on skin morphology, fur coat and sebaceous gland It was illustrated by the results that the follicular structure began to change from the next day after injection; keratinization occured in follicle and bulb of guard hair after the seventh day, and by the 15 th day, follicular structure began to recovered and raise up; guard hair could be easily pulled off during the 17 th to 20 th day that was thought the most suitable period for guard hair collection The molting mechanism is therefore considered that: molting dose of cyclophosphamide may bring components of follicle, bulb, hair papilla and sebaceous gland a reversible malnutrition that leads to a metabolic disturbance The consequence is structure changes, but not inflammation or injury Cells in above parts recover their reproducibility to form another normal structure after cyclophosphamide was dischanged