| CCB、蔗糖及成熟时间对山羊卵母细胞去核效果的影响 |
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| 引用本文： | 高立功,山 灵,刘凤军.CCB、蔗糖及成熟时间对山羊卵母细胞去核效果的影响[J].西北农林科技大学学报(社会科学版),2007,35(5):6-8. |
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| 作者姓名： | 高立功 山 灵 刘凤军 |
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| 作者单位： | 西北农林科技大学,生物工程研究所,陕西,杨凌,712100 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 分离培养山羊卵母细胞,采用盲吸法去核,研究不同成熟培养时间(18,22和26 h)不同细胞松弛素B(CCB)浓度(5,7.5,10,15 mg/L)和不同蔗糖浓度(20,25,30 g/L)对卵母细胞去核效果的影响。结果表明,山羊卵母细胞最佳去核条件为:卵母细胞体外成熟时间为22 h,去核时操作液中CCB质量浓度为7.5 mg/L,蔗糖质量浓度为20 g/L。
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| 关 键 词： | 山羊卵母细胞 细胞松弛素B 蔗糖 去核 核移植 |
| 文章编号： | 1671-9387（2007）05-0006-03 |
| 收稿时间： | 2006/3/14 0:00:00 |
| 修稿时间： | 2006-03-14 |
Effect of cytochalasin-B and sucrose and mature time on goat oocytes enucleation |
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| GAO Li-gong,SHAN Ling,LIU Feng-jun,ZHAO Xiao-e,ZHANG Yong.Effect of cytochalasin-B and sucrose and mature time on goat oocytes enucleation[J].Journal of Northwest Sci-Tech Univ of Agr and,2007,35(5):6-8. |
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| Authors: | GAO Li-gong SHAN Ling LIU Feng-jun ZHAO Xiao-e ZHANG Yong |
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| Institution: | (Institute of Bio-Engineering,Northwest A & F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | Goat oocytes were separated and cultured,and then enucleated under the different situation of maturing time,cytochalasin-B and sucrose concentration.The results showed that the optimum enucleation conditions were as follows:goat oocytes maturing time was 22 h;the cytochalasin-B concentration was 7.5 mg/L;the sucrose concentration was 25 g/L.Therefore,the best enucleation effects could be achiered by using cytochalasin-B together with sucrose. |
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| Keywords: | goat oocyte cytochalasin-B sucrose enucleation nuclear transfer |
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