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Significance of timing and level of inoculation with rhizosphere bacteria on wheat plants
Affiliation:1. Faculté des Sciences, Université Marien N’Gouabi, BP 69 Brazzaville, Congo;2. Microbiology Research Unit, School of Human Sciences, London Metropolitan University, London, N7 8DB, UK;3. Ouoba-Consulting, London, UK;1. Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín-Consejo Superior de Investigaciones Científicas (EEZ-CSIC), Profesor Albareda 1, 18008 Granada, Spain;2. Department of Plant Sciences, Laboratory of Molecular Biology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, Netherlands;1. School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China;2. Xi’an Key Laboratory of Textile Chemical Engineering Auxiliaries, School of Environmental and Chemical Engineering, Xi’an Polytechnic University, Xi’an 710000, China;1. Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India;2. Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India;3. Division of Agronomy, Indian Agricultural Research Institute, New Delhi 110012, India;1. ICAR-Indian Institute of Spices Research, P.O. Box 1701, Marikunnu P.O., Calicut 673012, Kerala, India;2. ICAR-Indian Agricultural Research Institute, New Delhi, India;3. Plant Pathology and Microbiology (PLPM), Texas A&M University, College Station, TX, USA
Abstract:The importance of time of inoculation and bacterial concentration in the inoculum on the response of wheat plants was evaluated, using eight strains of rhizosphere bacteria. The optimal bacterial concentration, for all strains, was 105–106 colony forming units ml−1. Plant response was highest when seeds had been inoculated but was less when seedlings were inoculated. Successive inoculations somewhat increased plant response. Early inoculations resulted in an increased colonization of plant roots at later stages of growth. It was concluded that time of inoculation and the concentration of bacteria in the inoculum were of significant importance in plant response to inoculation and they may govern the inconsistency found in inoculation experiments using beneficial bacteria.
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