| 兔出血症病毒单克隆抗体的抗原识别位点分析 |
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| 引用本文： | 孙智锋,杜念兴.兔出血症病毒单克隆抗体的抗原识别位点分析[J].南京农业大学学报,1997,20(1):68-72. |
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| 作者姓名： | 孙智锋 杜念兴 |
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| 作者单位： | 南京农业大学动物医学院 |
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| 摘 要： | 通过ＥＬＩＳＡ迭加试验对５株抗兔出血症病毒（ＲＨＤＶ）单克隆抗体（ＭｃＡｂ）的抗原表位进行了分析。抗体反应增值结果表明，６株单抗分别针对３类不同的抗原决定簇。ＣＧ４－１、ＣＨ３－１和ＡＡ８－１针对病毒结构多肽上的同一决定簇，ＣＦ２－１和ＲＭ－１７针对另一决定族，而ＲＭ－１４针对第３种决定簇；其识别表位可能与ＡＡ８－１非常接近。单抗的识别表位与其生物学活性密切相关。
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| 关 键 词： | 兔 出血症病毒 单克隆抗体 抗原识别位点 ELISA |
ANALYSIS OF THE ANTIGENIC EPITOPES RECOGNIZED BY MONOCLONAL ANTIBODIES TO RABBIT HEMORRHAGIC DISEASE VIRUS |
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| Sun Zhifeng,Du Nianxing and Xu Weiyan.ANALYSIS OF THE ANTIGENIC EPITOPES RECOGNIZED BY MONOCLONAL ANTIBODIES TO RABBIT HEMORRHAGIC DISEASE VIRUS[J].Journal of Nanjing Agricultural University,1997,20(1):68-72. |
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| Authors: | Sun Zhifeng Du Nianxing and Xu Weiyan |
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| Abstract: | Six strains of monoclonal antibodies (McAb) were confirmed to be specific for three different kinds of epitopes on rabbit hemorrhagic disease virus (RHDV) by competitive binding assay. Of them, AA8 1, CG4 1 and CH3 1 might direct to the same antigenic determinant on viral structural peptide, CF2 1 and RM 17 to another antigenic site, and RM 14 to the third epitope, which was close to that of AA8 1. These epitopes recognized by McAbs were consistent with their biological activities. |
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| Keywords: | rabbit hemorrhagic disease virus (RHDV) monoclonal antibodies (McAb) epitope enzyme linked immunosorbent assay (ELISA) |
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