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Serodiagnosis of equine infectious anemia by agar gel immunodiffusion and ELISA using a recombinant p26 viral protein expressed in Escherichia coli as antigen |
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| Authors: | Piza Adriana S Toledo Pereira Alessandra Rael Terreran Maria Thereza Mozzer Otto Tanuri Amílcar Brandão Paulo Eduardo Richtzenhain Leonardo José |
| Institution: | aDepartment of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, CEP 05508-270, São Paulo, SP, Brazil bLaboratory of Molecular Virology, Biosciences Institute, Federal University of Rio de Janeiro, CEP 21941-590, Rio de Janeiro, RJ, Brazil cVallée Laboratories, CEP 04571-000, São Paulo, SP, Brazil |
| Abstract: | We used a p26 recombinant protein (p26r) from equine infectious-anemia virus (EIAV) expressed in Escherichia coli as antigen to standardize an agar-gel immunodiffusion (AGIDp26r) test and an indirect ELISA (ELISAp26r) for the detection of antibodies against EIAV in 720 equine sera from Brazil. We evaluated the tests's relative diagnostic sensitivities (relSe) and relative diagnostic specificities (relSp) against a commercial AGID kit (Idexx?, USA). We used three sera panels: panel A—196 AGID-negative sera from an AIE non-endemic controlled area; panel B—194 AGID-negative sera from an AIE endemic area and panel C—330 AGID-positive sera from an AIE endemic area. ELISAp26r cut-off value was defined with TG-ROC using sera from panels A and C. AGIDp26r showed an agreement of 100% with the commercial kit. When applied to sera from panels A and C, ELISAp26r showed an agreement of 100% with the kit, but, although relSe was 100% for panel C, the ELISAp26r had relSp of 93.3%. |
| Keywords: | Equine infectious anemia ELISA AGID p26 |
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