| 白皮松天然群体遗传多样性的EST-SSR分析 |
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| 引用本文： | 赵罕,郑勇奇,李斌,张川红,林富荣,于雪丹,程蓓蓓,黄平. 白皮松天然群体遗传多样性的EST-SSR分析[J]. 林业科学研究, 2014, 27(4): 474-480 |
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| 作者姓名： | 赵罕 郑勇奇 李斌 张川红 林富荣 于雪丹 程蓓蓓 黄平 |
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| 作者单位： | 中国林业科学研究院林业研究所, 林木遗传育种国家重点实验室, 国家林业局林木培育重点实验室, 北京 100091;中国林业科学研究院经济林研究开发中心, 河南 郑州 450003;中国林业科学研究院林业研究所, 林木遗传育种国家重点实验室, 国家林业局林木培育重点实验室, 北京 100091;中国林业科学研究院林业研究所, 林木遗传育种国家重点实验室, 国家林业局林木培育重点实验室, 北京 100091;中国林业科学研究院林业研究所, 林木遗传育种国家重点实验室, 国家林业局林木培育重点实验室, 北京 100091;中国林业科学研究院林业研究所, 林木遗传育种国家重点实验室, 国家林业局林木培育重点实验室, 北京 100091;中国林业科学研究院林业研究所, 林木遗传育种国家重点实验室, 国家林业局林木培育重点实验室, 北京 100091;中国林业科学研究院林业研究所, 林木遗传育种国家重点实验室, 国家林业局林木培育重点实验室, 北京 100091;中国林业科学研究院林业研究所, 林木遗传育种国家重点实验室, 国家林业局林木培育重点实验室, 北京 100091 |
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| 基金项目： | 国家科技支撑计划课题（2013BAD01B06） |
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| 摘 要： | 为探讨白皮松群体间遗传变异规律,使用7对EST-SSR引物对分布区内21个白皮松天然群体的遗传多样性及遗传分化水平进行了研究。结果表明:7对引物在21个白皮松天然群体的663个单株中共检测到14个多态性位点。各群体间有效等位基因数(Ne)、Shannon’s信息指数(I)、观测杂合度(Ho)、期望杂合度(He)、Nei’s期望杂合度(Nei’s)分别为1.156 5 1.601 9、0.133 5 0.492 5、0.138 4 0.397 3、0.0860 0.342 8、0.084 6 0.337 4。白皮松群体间遗传分化系数(Fst)平均为0.215 2,基因流(Nm)值平均为0.911 9,群体间基因交流总体较少,遗传分化较大。白皮松多样性水平在分布区内呈规律性变化,多样性分布的中心区域主要在西部、南部,具有从西向东,从南向北依次减少的趋势。
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| 关 键 词： | 白皮松 天然群体 EST-SSR 遗传多样性 |
| 收稿时间： | 2012-06-05 |
Genetic Diversity Analysis of Pinus bungeana Natural Populations with EST-SSR Markers |
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| ZHAO Han,ZHENG Yong-qi,LI Bin,ZHANG Chuan-hong,LIN Fu-rong,YU Xue-dan,CHENG Bei-bei and HUANG Ping. Genetic Diversity Analysis of Pinus bungeana Natural Populations with EST-SSR Markers[J]. Forest Research, 2014, 27(4): 474-480 |
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| Authors: | ZHAO Han ZHENG Yong-qi LI Bin ZHANG Chuan-hong LIN Fu-rong YU Xue-dan CHENG Bei-bei HUANG Ping |
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| Affiliation: | Research Institute of Forestry, Chinese Academy of Forestry; State Key Laboratory of Tree Genetics and Breeding; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;Non-timber Forestry Research and Development Center, Chinese Academy of Forestry, Zhengzhou 450003, He'nan, China;Research Institute of Forestry, Chinese Academy of Forestry; State Key Laboratory of Tree Genetics and Breeding; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;Research Institute of Forestry, Chinese Academy of Forestry; State Key Laboratory of Tree Genetics and Breeding; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;Research Institute of Forestry, Chinese Academy of Forestry; State Key Laboratory of Tree Genetics and Breeding; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;Research Institute of Forestry, Chinese Academy of Forestry; State Key Laboratory of Tree Genetics and Breeding; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;Research Institute of Forestry, Chinese Academy of Forestry; State Key Laboratory of Tree Genetics and Breeding; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;Research Institute of Forestry, Chinese Academy of Forestry; State Key Laboratory of Tree Genetics and Breeding; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;Research Institute of Forestry, Chinese Academy of Forestry; State Key Laboratory of Tree Genetics and Breeding; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China |
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| Abstract: | In order to explore the genetic variation among Pinus bungeana populations, their genetic diversity and differentiation level were studied in 21 natural populations by EST-SSR markers. 14 polymorphic loci were detected in 663 individuals among 21 natural populations using 7 pairs of EST-SSR primes. The effective number of alleles (Ne), Shannon's information index (I), observed heterozygosity (Ho), expected heterozygosity (He) and Nei's expected heterozygosity (Nei's) were 1.156 5-1.601 9, 0.133 5-0.492 5, 0.138 4-0.397 3, 0.086-0.342 8, and 0.084 6-0.337 4 respectively. The results of genetic differentiation analysis showed that the average Fst and Nm were 0.215 2 and 0.911 9 respectively, the gene flow was less and the genetic differentiation was high among populations. Central regions of genetic diversity are mainly in west and south of the distribution and the genetic diversity in distribution changed regularly from west to east and from south to north. |
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| Keywords: | Pinus bungeana natural population EST-SSR genetic diversity |
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