| 胆盐水解酶基因在干酪乳杆菌LC2W中的表达 |
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| 引用本文： | 黄艳娜,刘振民,游春苹.胆盐水解酶基因在干酪乳杆菌LC2W中的表达[J].乳业科学与技术,2016,39(2):21-24. |
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| 作者姓名： | 黄艳娜 刘振民 游春苹 |
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| 作者单位： | 光明乳业股份有限公司,光明乳业研究院,乳业国家重点实验室,上海 200436 |
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| 基金项目： | “十二五”国家科技支撑计划项目(2013BAD18B01,2013BAD18B02) |
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| 摘 要： | 从胆盐水解酶的特性出发,在干酪乳杆菌LC2W中过量表达Lactobacillus plantarum ST-Ⅲ 4种胆盐水解酶同工酶,通过酶活性的测定研究胆盐水解酶对干酪乳杆菌水解胆盐的作用.结果表明:实现了4种胆盐水解酶在干酪乳杆菌LC2W的过表达;利用茚三酮显色法测定的4种重组菌胆盐水解酶活性较宿主LC2W都不同程度地降低;在干酪乳杆菌LC2W中过量表达L.plantarum ST-Ⅲ的胆盐水解酶基因达不到提高该菌水解胆盐能力的目的.
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| 关 键 词： | 胆盐水解酶 干酪乳杆菌LC2W 克隆表达 酶活性 |
Overexpression of Bile Salt Hydrolase Genes in Lactobacillus casei LC2W |
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| HUANG Yanna,LIU Zhenmin,YOU Chunping.Overexpression of Bile Salt Hydrolase Genes in Lactobacillus casei LC2W[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2016,39(2):21-24. |
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| Authors: | HUANG Yanna LIU Zhenmin YOU Chunping |
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| Institution: | (State Key Laboratory of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co. Ltd., Shanghai 200436, China) |
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| Abstract: | In this study, the recombinant Lactobacillus casei LC2W were constructed for overexpressing four bsh genes
from L. plantarum ST-III and the Bile salt hydrolase (BSH) enzyme activities were determined. It was shown that the four
recombinant strains all showed a lower enzyme activity than the host, indicating that the bsh gene overexpression could not
achieve the goal of enhancing the hydrolysis of the bile salts. |
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| Keywords: | bile salt hydrolase Lactobacillus casei LC2W gene cloning and overexpression enzyme activity |
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