| 高效液相色谱法测定乳粉中异构化乳糖的含量 |
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| 引用本文： | 刘丽君,李素琴,赵贞,岳虹,李翠枝,邵建波. 高效液相色谱法测定乳粉中异构化乳糖的含量[J]. 乳业科学与技术, 2016, 39(1): 11-13. DOI: 10.15922/j.cnki.jdst.2016.01.004 |
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| 作者姓名： | 刘丽君 李素琴 赵贞 岳虹 李翠枝 邵建波 |
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| 作者单位： | 内蒙古伊利实业集团股份有限公司,内蒙古呼和浩特,010110 |
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| 摘 要： | 建立乳粉中异构化乳糖高效液相色谱的测定方法.采用盐酸沉淀蛋白,氨基色谱柱分离,流动相为80％乙腈溶液,示差检测器检测.每100 g乳粉中异构化乳糖添加量为0.3～2.0 g时,回收率97.4％～103.0％.定量检出限为0.3 g/100 g.该方法操作简便,检测准确,可用于乳粉中异构化乳糖的检测.
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| 关 键 词： | 高效液相色谱法；异构化乳糖；乳粉 |
High Performance Liquid Chromatography Determination of Lactulose in Milk Powder |
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| LIU Lijun,LI Suqin,ZHAO Zhen,YUE Hong,LI Cuizhi,SHAO Jianbo. High Performance Liquid Chromatography Determination of Lactulose in Milk Powder[J]. JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY, 2016, 39(1): 11-13. DOI: 10.15922/j.cnki.jdst.2016.01.004 |
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| Authors: | LIU Lijun LI Suqin ZHAO Zhen YUE Hong LI Cuizhi SHAO Jianbo |
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| Affiliation: | (Inner Mongolia Yili Industrial Group Co. Ltd., Hohhot 010110, China) |
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| Abstract: | A method for the determination of lactulose in milk powder by high performance liquid chromatography (HPLC)was developed. Lactulose was extracted with water. The analysis was performed on a NH2 column using 80% acetonitrile inwater as the mobile phase. The recovery of lactulose spiked into milk powder in the range of 0.3–2.0 g/100 g was 97.4%–103.0%. The limit of detection was 0.3 g/100 g. The results indicated that this method is accurate, simple and suitable for theanalysis of lactulose in milk powder. |
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| Keywords: | high performance liquid chromatography (HPLC) lactulose milk powder, |
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