转NTHK1基因对杨树各器官中Na~+、K~+分布的影响

以盐渍生境下 1～2 年生 107 和 18-1 杨树为材料,对不同树龄和地径的杨树各营养器官中 Na+、K+分布变化进行了研究。结果表明,Na+、K+在杨树各营养器官中分布不同且差异显著。不同树龄和地径的 107 杨树的根、茎、叶部位的 Na+、K+含量变化都很明显,含NTHK1 的转基因 18-1 杨树根部的 Na+、K+含量基本保持恒定,茎、叶部位的 Na+含量变化均不明显,茎部的K+含量随着树龄的增加而升高,叶片部位的K+含量随着树龄的增加而降低。研究表明,在盐渍生境中种植的含 NTHK1 的转基因18-1 杨树比 107 杨树更加耐盐,可以更好地抵御盐胁迫,维持离子平衡,NTHK1 基因可能是通过增强转基因杨树聚集有害Na+至液泡的能力,以避免细胞质中过高的Na+对细胞造成伤害,从而提高了转基因植株的耐盐性。

Effect of Transgene NTHK1 on Distribution of Na~+ and K~+ in Different Organs of Poplar

Researched the distribution of Na+ and K+ in different vegetative organs of poplar material ‘107’ and ‘18-1’, which were 1~2 year old tree and different in ground diameter in saline environment. The results indicated that the distribution of Na + and K+ in poplar vegetative organs was different.The change of content of Na+ and K+ in roots, stems and leaves of ‘107’ was obvious with different tree age and different ground diameter ,the content of Na+ and K+ in roots of ‘18-1’ containing NTHK1 was fundamentally equal with different tree age and ground diameter. The content of K + in stems raised with increase of tree age,the content of K + in leaves lowered with increase of tree age,the content of Na+ in stems and leaves was lesser .The features that poplar ‘18-1’ was more applicable the saline than ‘107’,which can better resist to salt stress and maintain ion homeostasis. The effect of NTHK1 may be limitted a large number of Na+ in the vacuole and reduced ion toxicity of high consistency Na+ to cell, so the salt tolerance ability of 18-1 containing NTHK1 was enhanced.