响应面法优化超声波辅助提取石榴籽油的研究

采用超声波辅助有机溶剂法对3种不同品种石榴籽油进行提取。在单因素试验基础上,选择液料比、超声波功率、处理时间、处理温度为自变量,石榴籽油提取率为响应值,采用Box-Behnken试验设计方法,研究各自变量及其交互作用对石榴籽油提取率的影响。利用DesignExpert软件得到回归方程的预测模型并进行响应面分析。结果表明,提取温度、时间、液料比3个因素对石榴籽油提取率都有显著影响,温度和时间、液料比和其他3个因素的交互作用都有显著影响。确定超声波辅助提取石榴籽油的最佳条件为:提取温度39.93℃,时间33.20min,超声波功率346.24W,液料比11.40mL·g-1,此时提取率为96.48%。

Optimization on Ultrasonic-assisted Extraction Technology of Oil from Pomegranate Seeds with Response Surface Analysis

Ultrasonic-assisted extraction technology was used to extract seed oil from three different kinds of pomegranate. Based on the single factor analysis, the effect of liquid-solid ratio, ultrasonic power, extraction temperature and extraction time on enhancing oil yield from pomegranate seed was evaluated by response surface analysis. A 4-factor, 3-level Box-Behnken experimental design was applied and the interactions of each factor on the oil yield were also investigated. The results showed that the temperature, extraction time and liquid-solid ratio had significant effects on extraction yield, the interaction of extraction temperature and time was very significant, the interactions of liquid-solid ratio and other three factors were significant. The optimal conditions were at 39.93 ℃ extraction temperature, 11.40 mL·g-1 liquid-solid ratio, 346.24 W ultrasonic power, 33.20 min extraction time. Under such conditions, the oil yield was 96.48%.