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Shoot production through cutting culture and micrografting from mature tree explants inAcacia tortilis (Forsk.) Hayne subsp.raddiana (Savi) Brenan
Authors:C. Detrez
Affiliation:(1) Laboratoire de Microbiologie des Sols, ORSTOM Bel Air, B. P. 1386, Dakar, Senegal
Abstract:Shoot propagation from mature tree explants ofAcacia tortilis subsp.raddiana, a tropical tree legume, was studied. The effects of (i) the size of cuttings and (ii) a recut treatment of the donor trees, on axillary bud growth from multinodal horticultural cuttings are reported. Apices from seedlings, axillary buds from young lignified branches of mature trees, and axillary buds obtained through in vitro axillary branching from microcuttings isolated onto mature trees were all successfully micrografted on seedling rootstocks. A sterilization procedure for field explants is reported. Propagation via cuttings and via micrografting are discussed with regard to rejuvenation of mature donor trees.
Keywords:Acacia tortilis subsp.raddiana  multipurpose trees  propagation  in vitro micrografting  rejuvenation
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