Haemoplasma infection is not a common cause of canine immune-mediated haemolytic anaemia in the UK |
| |
Authors: | Warman S M Helps C R Barker E N Day S Sturgess K Day M J Tasker S |
| |
Institution: | School of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol BS40 5DU. |
| |
Abstract: | Objective : The aim of this study was to investigate whether the two canine haemoplasma species, Mycoplasma haemocanis and “Candidatus Mycoplasma haematoparvum,” are commonly associated with immune-mediated haemolytic anaemia (IMHA) in UK dogs. Methods : Three groups of dogs were recruited to the study: anaemic dogs with primary IMHA (n=37); anaemic dogs not meeting the inclusion criteria for primary IMHA (n=77) and non-anaemic dogs (n=113). DNA was extracted from 100 μl of blood and subjected to real-time quantitative polymerase chain reaction (qPCR) assays for both species of Mycoplasma. Each assay incorporated co-amplification of canine glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an endogenous internal control. Results : Canine GAPDH was successfully amplified by qPCR from all 227 canine blood samples but none contained M. haemocanis or “Candidatus M. haematoparvum” DNA. Clinical Significance : Haemoplasma infection is uncommon in dogs in the UK and no evidence was found that these organisms act as triggers for IMHA. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|