| HPLC测定蒲公英中槲皮素含量的研究 |
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| 引用本文： | 肖奇志.HPLC测定蒲公英中槲皮素含量的研究[J].安徽农业科学,2010,38(30):16871-16872. |
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| 作者姓名： | 肖奇志 |
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| 作者单位： | 贵阳护理职业学院,贵州贵阳,550003 |
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| 摘 要： | 目的]建立蒲公英中槲皮素含量的测定方法。方法]采用高效液相色谱法,AgilentC18柱（150.0mm×4.6mm,5.0μm）,流动相为甲醇-乙腈（63∶26）,紫外检测波长为370nm。结果]槲皮素在0.1～1.0mg/ml范围内线性关系良好,平均回收率为98.37%。结论]该方法分离好、快速、重复性好,可作为蒲公英的质量控制方法。
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| 关 键 词： | 蒲公英 槲皮素 高效液相色谱法 |
Determination of Quercetin Content in Dandelion by HPLC |
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| XIAO Qi-zhi.Determination of Quercetin Content in Dandelion by HPLC[J].Journal of Anhui Agricultural Sciences,2010,38(30):16871-16872. |
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| Authors: | XIAO Qi-zhi |
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| Institution: | XIAO Qi-zhi (Guiyang Nursing Vocational College,Guiyang,Guizhou 550030) |
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| Abstract: | Objective]The research aimed to establish a method for determining the content of quercetin in dandelion.Method]The content of quercetin in dandelion was determined by HPLC.The analysis was performed on Agilent C18 column(150.0 mm×4.6 mm,5.0 μm),with the mixture of methanol and acetonitrile(63:26) as mobile phase.The detection wave length was 370 nm.Result]The linear range of quercetin was 0.1-1.0 mg/ml.The average recovery was 98.37%.Conclusion]The method is simple,sensitive and accurate for HPLC to control the quality of dandelion. |
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| Keywords: | Dandelion Quercetin HPLC |
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