| 家蚕蛋白激酶C受体基因( Bmrack )的克隆及序列分析 |
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| 引用本文： | 刘艳艳,池旭娟,方向明,阚雪芹,苏媚,刘文倩,王海玲,谈建中.家蚕蛋白激酶C受体基因( Bmrack )的克隆及序列分析[J].蚕业科学,2009,35(3). |
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| 作者姓名： | 刘艳艳 池旭娟 方向明 阚雪芹 苏媚 刘文倩 王海玲 谈建中 |
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| 作者单位： | 苏州大学建筑与城市环境学院,江苏苏州,215123;苏州大学建筑与城市环境学院,江苏苏州,215123;苏州大学蚕桑研究所,江苏苏州,215123 |
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| 基金项目： | 国家高技术研究发展计划"863"项目 |
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| 摘 要： | 蛋白激酶C受体蛋白(RACK)除作为细胞内蛋白激酶C的受体之外,还作为细胞内重要的支架蛋白调节细胞功能。应用生物信息学方法对家蚕RACK蛋白的编码基因Bmrack进行电子克隆,获得了1224 bp的重组序列,基因编码区长960 bp,编码319个氨基酸,蛋白分子质量为36.04 kD,等电点为8.07。从家蚕幼虫脂肪体基因组DNA中PCR克隆了Bmrack基因,序列总长为2 122 bp,含有4个内含子和5个外显子,通过家蚕WGS比对,结果与其中5条序列的一致性为99%,且修复了其中8个缺口。聚类分析表明RACK蛋白的氨基酸序列在鳞翅目昆虫中非常保守。
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| 关 键 词： | 家蚕 蛋白激酶C受体基因 克隆 序列分析 |
Cloning and Sequence Analysis of Receptor for Activated Protein Kinase C(Bmrack) Gene in Silkworm,Bombyx mori |
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| LIU Yan-Yan,CHI Xu-Juan,FANG Xiang-Min,KAN Xue-Qin,SU Mei,LIU Wen-Qian,WANG Hai-Ling,TAN Jian-Zhong.Cloning and Sequence Analysis of Receptor for Activated Protein Kinase C(Bmrack) Gene in Silkworm,Bombyx mori[J].Acta Sericologica Sinica,2009,35(3). |
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| Authors: | LIU Yan-Yan CHI Xu-Juan FANG Xiang-Min KAN Xue-Qin SU Mei LIU Wen-Qian WANG Hai-Ling TAN Jian-Zhong |
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| Institution: | LIU Yan-Yan1 CHI Xu-Juan1 FANG Xiang-Ming1 KAN Xue-Qin1 SU Mei1 LIU Wen-Qian1 WANG Hai-Ling1 TAN Jian-Zhong1,2* (1School of Architecture , Urban Environment,Soochow University,Suzhou Jiangsu 215123,China,2Institute of Sericulture,China) |
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| Abstract: | Apart from acting as receptor for protein kinase C in cells,the receptor for activated protein kinase C(RACK) is also an important scaffold protein to have other regulatory functions in cells.In present study,Bombyx mori rack gene(Bmrack) was in silico cloned based on bioinformatics and a contig of 1 224 bp was obtained.The length of its coding region was 960 bp and coded for 319 amino acids.Its molecular weight was 36.04 kD and pI was 8.07.After that,Bmrack gene was cloned from larval fat body genomic DNA ... |
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| Keywords: | Bombyx mori rack gene Cloning Sequence analysis |
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