| 水稻光敏核不育基因pms3的精细定位 |
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| 引用本文： | 李香花,王伏林,陆青,徐才国.水稻光敏核不育基因pms3的精细定位[J].作物学报,2002,28(3):310-314. |
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| 作者姓名： | 李香花 王伏林 陆青 徐才国 |
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| 作者单位： | 华中农业大学作物遗传改良国家重点实验室,湖北武汉,430070 |
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| 基金项目： | 国家 8 63高科技计划 (Z16-0 2 -0 2 )，美国洛氏基金资助 |
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| 摘 要： | 为了进一步研究水稻晚粳品种农垦58转变为光敏核不育水稻农垦58S的突变位点,并精细定位光敏不育基因pms3,我们利用农垦58S/1514杂交组合的F1进行花药培养构建了一个DH群体,验证了在该群体中光敏不育受pms1、 pms3两对基因的控制,并根据分子标记分析选择第12染色体是1514基因型、 第7染色体农垦58S基因型的DH系DH80与农垦58S杂
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| 关 键 词： | 光敏核不育水稻 花药培养 偏分离 基因定位 RFLP标记 |
| 收稿时间： | 2001-04-16 |
| 修稿时间： | 2001年4月16日 |
Fine Mapping of PSGMS Gene pms3 in Rice (Oryza sativa L.) |
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| LI Xiang Hua WANG Fu Lin,LU Qing XU Cai Guo.Fine Mapping of PSGMS Gene pms3 in Rice (Oryza sativa L.)[J].Acta Agronomica Sinica,2002,28(3):310-314. |
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| Authors: | LI Xiang Hua WANG Fu Lin LU Qing XU Cai Guo |
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| Institution: | LI Xiang Hua WANG Fu Lin * LU Qing XU Cai Guo |
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| Abstract: | In order to determine the precise location of pms 3 gene which induce Nongken58(NK58) to PSGMS Nongken58S(NK58S), a DH population was constructed by culturing F 1 anthers of NK58S/1514. A line DH80, which was only with PSGMS gene pms1 , was selected to cross with NK58S for constructing a segregation population only in pms 3 locus. Segregation of RFLP markers was compared between DH and F 2 populations. And the results showed that several markers close to pms 3 on chromosome 12 normally segregated in F 2 population showed obviously distortion segregation in DH population. Meanwhile, some markers on chromosome 3, 7 and 11 in DH population also segregated distortedly. The genotypes for every F 3 family were determined by analysis of fertility segregation. And then the location of pms 3 and distances between RFLP markers and pms 3 were precisely calculated. The gene pms 3 is located between M36 and RZ261, but much closer to M36. |
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| Keywords: | PSGMS (photo sensitive genic male sterile) rice Anther culture Marker segregated distortion Gene location RFLPs |
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