欧洲鳗鲡免疫球蛋白阳性细胞的组织化学定位与分布特点

采用蛋白A亲和层析法分离纯化欧洲鳗鲡(Anguilla anguila,欧鳗)血清免疫球蛋白(Ig),制备其特异性兔抗血清,并运用免疫组织化学技术研究了嗜水气单胞菌(Aeromonas hydrophila)感染的试验组鳗鲡和未经感染的对照组鳗鲡脾脏、肾脏和肝脏Ig阳性(Ig )细胞定位与分布特点.结果表明:纯化后的Ig经SDS-PAGE检测含有分子质量约68 ku重链和26 ku轻链的2条清晰蛋白条带,由其制备的兔抗Ig血清效价达1:51 200.对照组欧鳗脾脏Ig 细胞数较少,肾脏相对较多,肝脏未发现.试验组欧鳗脾脏Ig 细胞数量明显比对照组增多,肾脏中有所增多但不明显,肝脏中未发现.在试验组和对照组中,黑色素巨噬细胞在脾脏和肾脏中均见明显聚集并与淋巴细胞形成黑色素巨噬细胞中心(melano-macrophage centres,MMCs),其中脾脏MMCs多数位于血管附近,脾脏Ig 细胞均主要分布在MMCs及其周边,且在试验组中数量多而密集,而肾脏Ig 细胞在对照组中明显分布于MMCs及其周边,但在试验组不明显.与对照组相比,试验组中脾脏和肾脏黑色素巨噬细胞数量和体积均有明显增多.试验结果说明欧鳗肾脏和脾脏是具有Ig 细胞的重要免疫器官,其中黑色素巨噬细胞数量和体积变化与免疫应答过程密切相关.

Immunocytochemical Localization and Distribution of Immunoglobulin Positive Cells in European Eel(Anguilla anguilla) Tissues

Purified immunoglobulin(Ig) was isolated from the serum of European eel by using protein A sepharose affinity chromatography.The polyclonal rabbit anti-eel IgM antiserum prepared by the purified Ig was then used to detect the localization and distribution of the immunoglobulin positive(Ig+) cells in the kidney,spleen and liver of the experimental(infected by Aeromonas hydrophila) and the control(not infected) European eels by the method of immunohistochemistry.Results of SDS-PAGE electrophoresis showed that...