| 核桃EST-SSR信息分析与标记的初步建立 |
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| 引用本文： | 朱艳,郝艳宾,王克建,吴春林,王维霞,齐建勋,周军.核桃EST-SSR信息分析与标记的初步建立[J].果树学报,2009,26(3). |
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| 作者姓名： | 朱艳 郝艳宾 王克建 吴春林 王维霞 齐建勋 周军 |
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| 作者单位： | 朱艳,周军,ZHU Yan,ZHOU Jun(西南林学院资源学院,昆明,650224);郝艳宾,王克建,吴春林,王维霞,齐建勋,HAO Yan-bin,WANG Ke-jian,WU Chun-lin,WANG Wei-xia,QI Jian-xun(北京农林科学院林业果树研究所,北京,100093)
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| 基金项目： | 北京市科技新星项目，西南林学院面上科研基金 |
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| 摘 要： | 利用生物信息学方法,从dbEST数据库用SSRHunter软件对4 500条核桃EST序列进行搜索,发现462个SSR分布于399条EST序列中,EST-SSR出现频率10.27%,平均每5.65 kb EST出现一个SSR。核桃二核苷酸重复基元占主导地位,其次为三核苷酸重复基元,分别占总SSR的49.57%和27.27%。核桃二核苷酸EST-SSR的优势类型为AG,其次为AT,分别占二核苷酸SSR的67.25%和28.38%。核桃三核苷酸EST-SSR的优势重复基元为AAG,其次为ACC、AAT、AGG,分别占三核苷酸SSR的25.40%、21.43%、16.67%和11.11%。用Primer Primer 5.0软件设计了11对引物,对核桃属5个样品进行PCR扩增,10对引物有扩增产物,引物有效扩增率90.9%,其中7对引物有多态性,多态性引物占可扩增引物的70%。表明,利用核桃EST序列开发EST-SSR标记是可行的。
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| 关 键 词： | 核桃 EST SSR信息 |
Analysis of SSRs information and development of SSR markers from walnut ESTs |
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| ZHU Yan,HAO Yan-bin,WANG Ke-jian,WU Chun-lin,WANG Wei-xia,QI Jian-xun,ZHOU Jun.Analysis of SSRs information and development of SSR markers from walnut ESTs[J].Journal of Fruit Science,2009,26(3). |
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| Authors: | ZHU Yan HAO Yan-bin WANG Ke-jian WU Chun-lin WANG Wei-xia QI Jian-xun ZHOU Jun |
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| Institution: | 1School of Resources Management;Southwest Forestry College;Kunming 650224 China;2Institute of Forestry and Pomology;Beijing Academy of Agriculture and Forestry Science;Beijing 100093 China |
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| Abstract: | 4 500 ESTs of walnut from the database of dbEST were screened using SSRHunter software,and 462 SSRs in 399 ESTs were mined out,accounting for 10.27% of the total ESTs,corresponding to one SSR in every 5.65 kb of the ESTs.Dinucleotide and trinucleotide repeats EST-SSRs were dominant,accounting for 49.57% and 27.27% respectively.AG was the most frequent motif in dinucleotide repeats,and the next was AT,accounting for 67.25% and 28.38% in dinucleotide EST-SSRs.AAG is the most frequent motif in trinucleotide re... |
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| Keywords: | EST |
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