Cloning,characterization, and expression analysis of a CC chemokine gene from turbot (Scophthalmus maximus)

The chemokines are a superfamily of chemotactic cytokines playing an important role in leukocyte chemotaxis. Here, a turbot head kidney cDNA library was constructed in which KC70 was identified as a CC chemokine. Unknown 5′ and 3′ parts of the cDNA were amplified by 5′ and 3′ rapid amplification of cDNA ends (RACE). The complete cDNA of KC70 contains a 59-bp 5′ UTR, a 336-bp ORF, and a 152-bp 3′ UTR. Four exons and three introns were identified in KC70. Phylogenetic analysis showed that KC70 was similar to CCL19. In normal turbot KC70 was expressed in all tissues except brain and skin. Infection of turbot with pathogenic bacteria significantly increased expression of KC70 in the liver. Expression of KC70 in head kidney first increased and then decreased after bacterial challenge. No significant change was observed in the spleen after bacterial challenge. During embryonic development, KC70 was highly expressed after the gastrula stage. These results indicated KC70 plays important and multiple roles in turbot immune response.