| 应用直接PCR技术快速筛查转基因玉米方法研究 |
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| 引用本文： | 邢珍娟,董立明,刘娜,夏蔚,李葱葱,李飞武. 应用直接PCR技术快速筛查转基因玉米方法研究[J]. 玉米科学, 2017, 25(1): 29-33 |
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| 作者姓名： | 邢珍娟 董立明 刘娜 夏蔚 李葱葱 李飞武 |
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| 作者单位： | 吉林省农业科学院农业质量标准与检测技术研究所, 长春 130033,吉林省农业科学院农业质量标准与检测技术研究所, 长春 130033,吉林省农业科学院农业质量标准与检测技术研究所, 长春 130033,吉林省农业科学院农业质量标准与检测技术研究所, 长春 130033,吉林省农业科学院农业质量标准与检测技术研究所, 长春 130033,吉林省农业科学院农业质量标准与检测技术研究所, 长春 130033 |
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| 基金项目： | 吉林省农业科技创新工程项目（2013） |
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| 摘 要： | 以转CP4-epsps基因玉米为研究对象,用玉米内标准基因z SSIIb及3种常见转基因成分(Ca MV35S启动子、NOS终止子、CP4-epsps基因)为检测靶标,建立基于直接PCR技术的玉米转基因成分筛查方法。此方法不用提取纯化DNA,只需取直径约为0.5 mm的叶片加到PCR反应体系中进行扩增,与常规PCR方法相比,在保证结果准确性的同时,大大缩短检测时间,提高工作效率。此方法具有操作简便、结果可靠等优点,适用于玉米叶片中转基因成分的快速筛查。
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| 关 键 词： | 转基因玉米 直接PCR 快速筛查 |
| 收稿时间： | 2016-10-14 |
Rapid Screening of Genetically Modified Maize by Direct PCR |
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| XING Zhen-juan,DONG Li-ming,LIU N,XIA Wei,LI Cong-cong and LI Fei-wu. Rapid Screening of Genetically Modified Maize by Direct PCR[J]. Journal of Maize Sciences, 2017, 25(1): 29-33 |
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| Authors: | XING Zhen-juan DONG Li-ming LIU N XIA Wei LI Cong-cong LI Fei-wu |
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| Affiliation: | Institute of Agricultural Quality Standard and Testing Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, China,Institute of Agricultural Quality Standard and Testing Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, China,Institute of Agricultural Quality Standard and Testing Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, China,Institute of Agricultural Quality Standard and Testing Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, China,Institute of Agricultural Quality Standard and Testing Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, China and Institute of Agricultural Quality Standard and Testing Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, China |
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| Abstract: | The transgenic maize with CP4-epsps gene was selected as the research material to detect the endogenous reference gene zSSIIb and three common transgenic elements(CaMV35S promoter, NOS terminator and CP4-epsps gene), through which a screening method for maize transgenic components based on direct PCR was established. Using this method, there was no need to extract and purify the DNA, only need to take 0.5mm leaves in diameter and added it to the PCR reaction system for amplification. Compared to conventional PCR method, the developed direct PCR method greatly shortened the working hours, and improved work efficiency, as well as ensuring the accuracy of the results. The method has the advantages of simple operation and reliable result, and is suitable for rapid screening of transgenic components in maize leaves. |
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| Keywords: | Genetically modified maize Direct PCR Rapid screening |
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