竹荪多糖的分离纯化和鉴定

中国福建古田人工栽培的竹荪（Ｄｉｃｔｙｏｐｈｏｒａｉｎｄｕｓｉａｔａ）子实休干品经热水提取，乙醇沉淀，蛋白法和Ｓｅｖａｇ法相结合去蛋白，ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－２５和ＳｅｐｈａｄｅｘＧ－２００柱纯化，得到竹荪多糖，命名为ＤＩ，用聚丙烯酰胺凝胶电泳和葡聚糖凝胶柱层析分析表明：该多糖为均一物质。竹荪多糖的分子量为１４４，０００，经紫外扫描，元核酸和蛋白质的特征吸收峰，红外扫描表明有典型的多糖吸收峰。该多糖经纸层析和气相色谱分析表明：其单糖组成为Ｌ—岩藻糖、Ｄ—木糖、Ｄ—甘露糖、Ｄ—葡萄糖，摩尔比为０．１６：０．２４：１．００：１．０８。

Isolation ,Purification and Identification of the Polysaccharide Dl from the Fruit Bodies of Dictyophora indusiata

A polysaccbaride ID was isolated from the fruit bodies of Dictyophora indusiata,itwas further purified by DBAE-Sephadex A-25 and Sephadex G-200,its homogeneity wasverified by gelfiltration chromatography and polyacrylamide gel electrophoresis.The com-ponents of DI were identified by paper and gas chromatography.It vas composed ofL-fucose,D-xylose,D-mann,D-glucose in the molar ratio of 0.16 : 0.24: 1.00: 1.08.Itsmolecular weight was estimated to be 144000.