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抗寒苹果“新冠”的茎尖组织培养
引用本文:张虎平,张志明,樊宪伟,张录霞,马兵钢. 抗寒苹果“新冠”的茎尖组织培养[J]. 北方园艺, 2009, 0(2)
作者姓名:张虎平  张志明  樊宪伟  张录霞  马兵钢
作者单位:1. 石河子大学,农学院园艺系,新疆,石河子,832003
2. 广西亚热带生物资源保护利用重点实验室,广西大学生命科学与技术学院,广西,南宁,530005
基金项目:石河子大学自然科学与技术创新基金 
摘    要:以苹果"新冠"茎尖为材料,通过研究不同的激素配比对芽增殖和生根的影响,探索抗寒苹果"新冠"的最适增殖培养基和生根培养基.结果表明:"新冠"的最适诱导培养基为MS+0.1 mg/L NAA+1.5 mg/L 6-BA;最适增殖培养基为MS+1.0 mg/L 6-BA+0.05或0.08 mg/LNAA,其增殖系数达到7.4;最适生根培养基为1/2MS+0.2 mg/L IBA+0.6 mg/L IAA,其生根率达到90.5%.

关 键 词:新冠苹果  茎尖组织培养  增殖培养  生根培养

Shoot-Tip Tissue Culture of Cold-Resistant Malus Xinguan
ZHANG Hu-ping,ZHANG Zhi-ming,FAN Xian-wei,ZHANG Lu-xia,MA Bing-gang. Shoot-Tip Tissue Culture of Cold-Resistant Malus Xinguan[J]. Northern Horticulture, 2009, 0(2)
Authors:ZHANG Hu-ping  ZHANG Zhi-ming  FAN Xian-wei  ZHANG Lu-xia  MA Bing-gang
Affiliation:1.Department of Horticulture;College of Agriculture;Shihezi University;Shihezi;Xinjiang 832003;China;2.Guangxi Key Laboratory Subtropical Bioresources Conservation and Utilization;College of Life Science and Technology;Guangxi University;Nanning;Guangxi 530005;China
Abstract:The effect of different phytohormone ration on the multiplication and rooting of shoot-tip explants from Malus Xinguan were studied.The results indicated that the most suitable induced medium was MS+0.1 mg/L NAA+1.5 mg/L 6-BA;the most suitable multiplication medium was MS+1.0 mg/L 6-BA+0.05 or 0.08 mg/L NAA,the multiplication index was 7.4;the best rooting media was 1/2MS+0.2 mg/L IBA+0.6 mg/L IAA,the rooting rate was 90.5%.
Keywords:Malus Xinguan  Shoot-tip tissue culture  Multiplication culture  Rooting culture  
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