| 抗寒苹果“新冠”的茎尖组织培养 |
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| 引用本文： | 张虎平,张志明,樊宪伟,张录霞,马兵钢.抗寒苹果“新冠”的茎尖组织培养[J].北方园艺,2009(2). |
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| 作者姓名： | 张虎平 张志明 樊宪伟 张录霞 马兵钢 |
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| 作者单位： | 1. 石河子大学,农学院园艺系,新疆,石河子,832003
2. 广西亚热带生物资源保护利用重点实验室,广西大学生命科学与技术学院,广西,南宁,530005 |
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| 基金项目： | 石河子大学自然科学与技术创新基金 |
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| 摘 要： | 以苹果"新冠"茎尖为材料,通过研究不同的激素配比对芽增殖和生根的影响,探索抗寒苹果"新冠"的最适增殖培养基和生根培养基.结果表明:"新冠"的最适诱导培养基为MS+0.1 mg/L NAA+1.5 mg/L 6-BA;最适增殖培养基为MS+1.0 mg/L 6-BA+0.05或0.08 mg/LNAA,其增殖系数达到7.4;最适生根培养基为1/2MS+0.2 mg/L IBA+0.6 mg/L IAA,其生根率达到90.5%.
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| 关 键 词： | 新冠苹果 茎尖组织培养 增殖培养 生根培养 |
Shoot-Tip Tissue Culture of Cold-Resistant Malus Xinguan |
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| ZHANG Hu-ping,ZHANG Zhi-ming,FAN Xian-wei,ZHANG Lu-xia,MA Bing-gang.Shoot-Tip Tissue Culture of Cold-Resistant Malus Xinguan[J].Northern Horticulture,2009(2). |
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| Authors: | ZHANG Hu-ping ZHANG Zhi-ming FAN Xian-wei ZHANG Lu-xia MA Bing-gang |
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| Institution: | 1.Department of Horticulture;College of Agriculture;Shihezi University;Shihezi;Xinjiang 832003;China;2.Guangxi Key Laboratory Subtropical Bioresources Conservation and Utilization;College of Life Science and Technology;Guangxi University;Nanning;Guangxi 530005;China |
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| Abstract: | The effect of different phytohormone ration on the multiplication and rooting of shoot-tip explants from Malus Xinguan were studied.The results indicated that the most suitable induced medium was MS+0.1 mg/L NAA+1.5 mg/L 6-BA;the most suitable multiplication medium was MS+1.0 mg/L 6-BA+0.05 or 0.08 mg/L NAA,the multiplication index was 7.4;the best rooting media was 1/2MS+0.2 mg/L IBA+0.6 mg/L IAA,the rooting rate was 90.5%. |
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| Keywords: | Malus Xinguan Shoot-tip tissue culture Multiplication culture Rooting culture |
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