甘蔗种质资源抗宿根矮化病特性鉴定

[目的]筛选抗甘蔗宿根矮化病(RSD)种质资源材料,为选育抗RSD甘蔗品种提供优良抗原.[方法]采用浸蔗种的方法对6个系列100份甘蔗品种进行侵染,用建立的RSD-PCR检测方法进行检测,计算各甘蔗品种RSD发病率,并对甘蔗种质资源进行抗性评价.[结果]100份甘蔗品种材料中,GT02/351、CP89/2134、ROC26、粤94/28、福农89/209、崖96/45等品种RSD发病率为0,表现为A级抗性;GT99/181、GT97/228、GT90/95等品种RSD发病率等于10%,表现为B级抗性;GT02/208、GT96/156、ROC22等品种表现为C级抗性;GT21、GT02/237、CP88/1762等品种表现为D级抗性.6个不同系列中,CP系列RSD平均发病率最低,为12.00%,抗性最强;粤糖系列RSD平均发病率最高,为28.57%,抗性较弱.[结论]GT02/351、CP89/2134、ROC26、粤94/28、福农89/209、崖96/45等甘蔗品种具有良好的抗性,具有推广应用价值;CP系列的抗性较强,可作为抗RSD的育种材料.

Ratoon stunting disease resistance evaluation on sugarcane germplasm resources

【Objective】This research screened out sugarcane ratoon stunting disease （RSD） germplasm resources in order to breed better sugarcane varieties with excellent RSD resistance trait. 【Method】By infusing 100 sugarcane varieties from 6 series using the sugarcane seedling-soaking method， the varieties were tested with the established PSD-PCR detection method， the RSD incidence rate in the sugarcane varieties were calculated， and the sugarcane germplasm resources underwent resistance evaluation. 【Result】Within the 100 tested sugarcane varieties， the RSD incidence rates of GT02/351， CP89/2134， ROC26， Yue 94/28， Funong 89/209， and Ya 96/45 were 0% and were rated with grade A resistance； the RSD incidence rates of GT99/181， GT97/228， and GT90/95 were less than 10% and were rated with grade B resistance. GT02/208， GT96/156， and ROC22 were all rated with grade C resistance， while GT21， GT02/237， and CP88/1762 were all rated with grade D resistance. Within the 6 different series， the RSD incidence rate of CP series averaged the lowest at 12%， therefore exhibiting the strongest resistance； the RSD incidence rate of Yuetang series averaged the highest at 28.57%， therefore exhibiting the weakest resistance. 【Conclusion】The sugarcane varieties GT02/351， CP89/2134， ROC26， Yue 94/28， Funong 89/209， and Ya 96/45 displayed excellent resistance quality， thereby demonstrated widespread application values. Within all the sugarcane series， the CP series showed the strongest resistance and could be used as anti-RSD breeding material.