氯吡脲和多效唑配施对山药叶片一些生理特性的影响

[目的]研究喷施氯吡脲和多效唑对山药叶片一些生理特性的影响,为阐明氯吡脲和多效唑配施提高山药块茎产量机理提供理论依据.[方法]以山药品种“桂淮2号”为材料,在块茎膨大初期进行氯吡脲和多效唑配施,测定山药叶片叶绿素、可溶性蛋白、可溶性糖、MDA、脯氨酸含量和POD活性变化,进行回归方程和差异显著性检验.[结果]氯吡脲和多效唑配施可提高山药叶片叶绿素和可溶性蛋白含量,降低叶片可溶性糖含量,叶片POD活性增强,脯氨酸积累增多,MDA降低,其中以氯吡脲影响较大.氯吡脲和多效唑配施的最适浓度分别为30、400 mg/kg.[结论]氯吡脲和多效唑适宜配施能够促进山药叶绿素合成,提高山药叶片蛋白质积累和增强抗性.

Effects of forchlorfenuron and paclobutrazol combination on the physiological characteristics of yam leaves

【Objective】This study aimed to understand the physiological characteristics of yam （Dioscorea opposita Thunb.） leaves after spraying the leaves with paclobutrazol and forchlorfenuron in order to provide theoretical basis for explaining the mechanism of forchlorfenuron and paclobutrazol combination on improving the yam tuber yield. 【Method】Using the yam variety “Guihuai 2” as testing material， paclobutrazol combined with forchlorfenuron was applied to the yam during the in-tuber expansion period to determine the chlorophyll content， soluble protein， soluble sugar， MDA， proline content， and changes in POD activity in yam leaves and to conduct the regression equation and difference test analysis. 【Result】Forchlorfenuron and paclobutrazol combination could improve the chlorophyll content and soluble protein content， reduce the soluble sugar content， strengthen the POD activity， increase the proline accumulation， and reduce the MDA content in yam leaves； out of the two fertilizers， the influences of forchlorfenuron were greater. The optimal application concentration for forchlorfenuron and paclobutrazol was 30 and 400 mg/kg， respectively. 【Conclusion】Appropriate concentrations of forchlorfenuron and paclobutrazol could promote the chlorophyll synthesis and improve the protein accumulation and resistance ability in yam leaves.