| 野桑蚕羧酸酯酶基因cDNA片段的克隆及序列分析 |
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| 引用本文： | 宋宏图,李兵,王东,赵华强,王文兵,沈卫德.野桑蚕羧酸酯酶基因cDNA片段的克隆及序列分析[J].安徽农业科学,2007,35(14):4112-4114. |
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| 作者姓名： | 宋宏图 李兵 王东 赵华强 王文兵 沈卫德 |
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| 作者单位： | 苏州大学蚕桑研究所,苏州大学生命科学学院,江苏苏州,215123;江苏大学生命科学学院,江苏镇江,212013 |
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| 基金项目： | 国家“973”计划（2005CB121005）;国家自然基金项目（30471309）. |
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| 摘 要： |
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| 关 键 词： | 野桑蚕 羧酸酯酶 克隆 RT-PCR |
| 文章编号： | 0517-6611（2007）14-04112-03 |
| 收稿时间： | 2007-02-10 |
| 修稿时间： | 2007-02-10 |
Cloning and Sequence Analysis of cDNA Fragment of Carboxylesterase Gene in Bombyx mandarina M. |
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| SONG Hong-tu et al.Cloning and Sequence Analysis of cDNA Fragment of Carboxylesterase Gene in Bombyx mandarina M.[J].Journal of Anhui Agricultural Sciences,2007,35(14):4112-4114. |
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| Authors: | SONG Hong-tu |
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| Institution: | Institute of Serieulture, School of Life Science, Suzhou University, Jiangsu, Suzhou 215123 |
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| Abstract: | RT-PCR technology was used to clone a cDNA fragment of carboxylesterase gene in Bombyx mandarina M.The length of the cDNA fragment was 427 bp and the deduced amino acid resides was 142.PCR result in different issues at the same growth period showed that except silk gland,there were fragments in the ovarian tubule,the hemacytopoietic organ,the fat body and the midgut.After the homologous comparison with Bombyx mori.and other insect esterases,the Bombyx mandarina M.carboxylesterase shared the highest homology(98.6 %) with Bombyx mori.carboxylesterase and lower homology(23.4 % ~ 30.7 %) with Tribolium castaneum. |
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| Keywords: | Bombyx mandarina M Carboxylesterase Clone RT-PCR |
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