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<Emphasis Type="Italic">Tobacco mosaic virus</Emphasis> Is Transmissible from Tomato to Tomato by Pollinating Bumblebees
Authors:Kiyotsugu OKADA  Shin-ichi KUSAKARI  Mitsuo KAWARATANI  Jun-ichi NEGORO  Satoshi T OHKI  Takeshi OSAKI
Institution:(1) Osaka Prefectural Agricultural and Forestry Research Center, Habikino 587-0862, Japan, JP;(2) College of Agriculture, Osaka Prefecture University, Sakai 599-8531, Japan, JP
Abstract:Tobacco mosaic virus (TMV) was detected by ELISA, electron microscopy and/or bioassay from bumblebee (Bumbus terrestris), pollen clumps, nest materials and bee-visited anthers of flowers from greenhouses in which tomatoes had been pollinated by bees and were severely infected with TMV. Experimental bee-mediated transmission of TMV in greenhouse tomatoes demonstrated that the bumblebees transported TMV from plant to plant and that they spread the virus in greenhouses. This is the first report describing TMV transmission by bumblebees. Received 11 August 1999/ Accepted in revised form 30 September 1999
Keywords:: Tobacco mosaic virus  bumblebee  transmission  tomato  
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