高氨氮降解菌株DA-1的分离鉴定及降解性能研究

一株高效的氨氮降解菌株DA-1从沼液中分离,16S rDNA序列分析将其鉴定为Arthrobacter arilaitensis。实验研究了不同碳源、C/N比及氨氮浓度对菌株DA-1氨氮降解性能的影响。结果表明:多种有机碳源条件下,当C/N比为10、菌液接种量为1%(v/v)时,在2 d内,菌株DA-1的氨氮降解率达到了80%以上(100 mg/L),随着氨氮浓度的上升降解率随之下降,当氨氮浓度高达1000 mg/L,降解率下降至26.3%。实验还考察了菌株DA-1对沼液样品的氨氮降解能力,通过添加乙醇调节沼液的C/N比,处理5 d后,沼液样品的氨氮浓度下降了70%以上。

Isolation, Identification and Degradation Ability of A High- efficient Ammonia - degrading Strain DA - 1

A high-efficient ammonia-degrading strain DA-1 was isolated from the anaerobically digested wastewater,and it was identified as Arthrobacter arilaitensis by 16S rDNA sequence analysis.Under the conditions of different carbon sources,C/N ratios and ammonia concentrations,the ammonia degradation ability of DA-1 was studied.The results showed that: when the C/N ratio was 10 and the inoculation amount of DA-1 was 1%(v/v),the degradation rate of 100 mg/L ammonia by this strain could exceed 80% within 2 days.Along with the increase of the ammonia concentration,the ammonia degradation rate decreased,when the ammonia concentration was up to 1000 mg/L,the degradation rate decreased to 26.3%.The ammonia degradation ability of DA-1 to the anaerobically digested wastewater samples was also investigated by regulating C/N ratio with ethanol,the ammonia concentration of the anaerobically digested wastewater sample was decreased by over 70% after being treated for 5 days.