黄花菜受精过程的研究

 利用常规石蜡切片技术对黄花菜受精过程进行了详细的研究, 获得如下结果: 1. 授粉后1～2 h花粉在柱头上萌发长出花粉管, 只有生殖细胞进入花粉管内。2. 花粉管在花柱道内生长历经20～22 h,生殖细胞分裂形成2个精子。3. 花粉管在子房室中轴胎座表面生长经历2～4 h。4. 授粉后26～28 h花粉管进入1个助细胞, 释放2个精子。5. 精卵融合经历4～6 h, 形成合子。6. 精核与次生核融合经历2～4 h, 形成初生胚乳核。7. 自花粉在柱头萌发至合子形成, 受精过程持续总时间为29～36 h。8. 合子分裂发生在授粉后39～48 h。9. 讨论了研究结果对黄花菜遗传育种与分子育种的意义。

Studies on Fertilization of Hemerocallis citrina Baroni

Observed the process of fertilization in Hemerocallis citrina Baroni in detail by means of paraffin wax.The main results are as follows:1.After artificial pollination 1-2 hours,pollens germinated at the top of stigma,growing out pollen tube,where only generative cell can get into.2.Pollen tube prolongs in stylar canal for 20-22 hours,in concomitant with generative cell dividing into two sperms.3.Pollen tube prolongs on the surface of axile placenta in locule for 2-4 hours.4.After artificial pollination 26-28 hours,pollen tube get into one of synergids,and releases two sperms.5.It takes 4-6 hours to eggsperm fusion,and then bring out zygocyte.6.The formation of primary endosperm nucleus results from 2-4 hours' egg-secondary nucleus fusion.7.The duration of the whole fertilization is 29-36 hours from pollen germination to the formation of zygocyte.8.After artificial pollination 39-48 hours,zygocyte begins to divide.9.Discussed the significance of the result of study to genetic breeding and molecular breeding.