| 化学诱变选育高产纤维素酶菌株 |
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| 引用本文： | 冯培勇,葛宜和,杨立红,张婷.化学诱变选育高产纤维素酶菌株[J].安徽农业科学,2008,36(33). |
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| 作者姓名： | 冯培勇 葛宜和 杨立红 张婷 |
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| 作者单位： | 鲁东大学生命科学学院,山东烟台,264025 |
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| 基金项目： | 鲁东大学校基金项目
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| 摘 要： | 目的]研究化学诱变剂对高产纤维素酶生产菌的选育。方法]以1株绿色木霉(Trichoderma virideF1)为出发菌株,经过亚硝基胍、硫酸二乙酯和氯化锂诱变处理,选育纤维素酶高产菌株生产菌。结果]经LiCl诱变后,得到1株产酶活力较高的菌株L6,相对酶活较出发菌株提高了35.7%。结论]化学诱变是诱变微生物、筛选优良菌株的有效手段。
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| 关 键 词： | 纤维素酶 化学诱变 绿色木霉 |
Breeding of High Cellulase Yielding Strain Induced by Chemical Mutagens |
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| Abstract: | Objective] The aim was to study the breeding of high cellulase yielding strain induced by chemical mutagens.Method] One strain of Trichoderma viride F1 was taken for original strain.After treated with NTG,DES and LiCl,the high cellulase-producing strain named L6 was bred.Result] Under suitable conditions,the CMCase activity of L6 increased by 35.7% compared with that of the original strain.Conclusion] Chemical mutation was the effective means to induce microorganism and breed superior strain. |
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| Keywords: | Cellulase Chemical mutagens Trichoderma viride |
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