| 定量PCR技术检测转基因玉米的研究 |
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| 引用本文： | 吴影,古绍彬.定量PCR技术检测转基因玉米的研究[J].农产品加工．学刊,2007(10):4-6. |
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| 作者姓名： | 吴影 古绍彬 |
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| 作者单位： | 河南科技大学,食品与生物工程学院,河南,洛阳,471003 |
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| 基金项目： | 河南科学大学人才引进基金 |
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| 摘 要： | 以Bt176转基因玉米为材料,通过使用特异性引物和染料,对转基因玉米中外源基因cry1Ab进行了定量检测。研究发现,在优化检测体系的基础上,SYBR荧光染料法定量检测转基因玉米,具有更高的检测灵敏度和精确度,其检测阈值可降到0.05%,变异系数为0.09%~0.53%。
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| 关 键 词： | 实时荧光PCR 转基因玉米 SYBR染料 |
| 文章编号： | 1671-9646（2007）10-0004-03 |
| 修稿时间： | 2007-08-06 |
Research on Detecting Genetically Modified Soybean by Real-time PCR Assay |
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| Wu Ying,Gu Shaobin.Research on Detecting Genetically Modified Soybean by Real-time PCR Assay[J].Nongchanpin Jlagong.Xuekan,2007(10):4-6. |
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| Authors: | Wu Ying Gu Shaobin |
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| Abstract: | A quantitative method was developed for detecting genetically modified maize by real-time PCR with the specific primer and fluorescence dye to amplify the exogenous crylAb gene. The standard curve of Ct vs the GM content in the reference materials was generated and a linear regression equation was obtained to quantity GM maize. The results showed that the quantitative method is very sensitive and precisive. |
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| Keywords: | real-time PCR Genetically modified maize SYBR quantitative detection |
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