黑曲霉产β-磷酸甘油酯酶固体发酵条件的研究

对一株高产β-磷酸甘油酯酶的黑曲霉进行最适培养方法和培养条件的研究。分别研究了底物浓度、氮源、培养基的含水量,以及培养基中的米糠和玉米粉的比例对黑曲霉产β-磷酸甘油酯酶的影响。实验结果表明,固体培养基中,用纱布覆盖培养基更适合黑曲霉产β-磷酸甘油酯酶的发酵,覆盖单层纱布的效果优于双层纱布。底物浓度在0.15%时,黑曲霉产酶活性最高。培养基中最佳米糠和玉米粉比例为6∶2。8g(干质量)培养基中的加水体积为21.5mL左右时,最利于黑曲霉产β-磷酸甘油酯酶的发酵。培养基中无机氮优于有机氮,且以硫酸氨最优。黑曲霉培养120h后再进行低温(4℃)处理72h的产酶活性最高。接种时搅拌和培养24h后的翻曲对黑曲霉产β-磷酸甘油酯酶都有促进作用,且接种时将菌种与培养基拌匀对产酶效率的提高比较明显。

Studies of Solid Ferments Condition on the Glycerol β-phosphatase of Aspergillus Niger

This paper has studied the best culture ways and conditions on Aspergillus Niger with high yield glycerol β-phosphatase. Specially, studied on the effects of concentration of substrate,nitrogen source,water capacity and the proportion of nice bran to cornmeal. The experiments results follow as:single gauze cover the medium,the concentration of substrate is 0.15%,the proportion of rice bran to cornmeal is 6∶2,21.5 mL water is put in 8 g medium(dry weight),(NH4)2SO4 is the nitrogen source,stirs when inoculation,the medium is put into 4 ℃ refrigerator for 72 h after culture of 120 h,it's good for glycerol β-phosphatase production.