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| 旋毛虫新生幼虫期特异性基因糖蛋白表达载体的构建 | |
| 引用本文： | 徐晓立,刘明远,卢强,任瑞文.旋毛虫新生幼虫期特异性基因糖蛋白表达载体的构建[J].塔里木农垦大学学报,2002,14(3):1-4. |
| 作者姓名： | 徐晓立 刘明远 卢强 任瑞文 |
| 作者单位： | 1. 塔里木农垦大学动物科技学院,新疆,阿拉尔,843300 2. 解放军军需大学动科系,吉林,长春,130062 3. 广州军区连勤部军事医学研究所,广东,广州,510507 |
| 摘 要： | 根据旋毛虫新生幼虫（NBL）期特异性全长cDNA SSC1基因序列，PCR扩增目的基因，克隆人pMD18T后，根据其读码框架，克隆人表达载体pET28b，构建其原核表达载体pET28b-SSC1。分别用Sal I和Xho I、Sma I和Hind Ⅲ双酶切；XbaI单酶切鉴定重组子，结果均与预期相符，进一步测序结果也表明重组载体读码框架正确，为进一步亚克隆和表达奠定基础。 |
| 关 键 词： | 糖蛋白表达载体 旋毛虫新生幼虫 期特异性基因 旋毛虫病 |
| 文章编号： | 1009-0568(2002)03-0005-03 |
| 修稿时间： | 2001年9月26日 |
Express Vector Construction of Trichinella Spiralis Newborn Larvae Stage-specific cDNAs |
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| Xu Xiaoli , Liu Mingyuan , Lu Qiang , Ren Ruiwen.Express Vector Construction of Trichinella Spiralis Newborn Larvae Stage-specific cDNAs[J].Journal of Tarim University of Agricultural Reclamation,2002,14(3):1-4. | |
| Authors: | Xu Xiaoli Liu Mingyuan Lu Qiang Ren Ruiwen |
| Institution: | Xu Xiaoli 1, Liu Mingyuan 2, Lu Qiang 2, Ren Ruiwen 3 |
| Abstract: | Based on the open reading frame of full length SSC1 which has been identified as NBL stage-specific gene coding an glycoprotein,the forward PCR primer (AGGTCGACGTTGCAACATGCAAAAACG)with a Sal I enzyme site has been designed using the software DNAsis. Using the forward primer and T7 primer, the full length gene of SSC1 was amplified. After cloned in pMD18 T Vector and digested with the enzyme Sal I and Xho I,and digested pET28b with Sal I, Xho I and CIAP, the stage specific gene SSC1 was recombined with the T7 promoter-driven expression vector pET28b.The identified with Enzyme digested and sequenced results consistent with designed,which suggested it can be used as express vector. |
| Keywords: | NBL stage-specific gene express vector |
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