Effect of individual male variability on cryopreservation of northern pike, Esox lucius L., sperm |
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| Authors: | I Babiak J Glogowski M J Luczynski M Luczynski |
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| Affiliation: | Department of Animal Biochemistry, Olsztyn University of Agriculture and Technology, Olsztyn, Poland;Inland Fisheries Institute, Olsztyn, Poland;Department of Basic Fishery Sciences, Olsztyn University of Agriculture and Technology, Olsztyn, Poland |
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| Abstract: | Milt from individual males of northern pike, Esox lucius L., was separately cryopreserved. Concentration of spermatozoa in fresh milt and spermatozoa motility before freezing and after thawing was evaluated. Activity of aspartate aminotransferase (AspAT, E.C. 2.6.1.1.) and acid phosphatase (AcP, E.C. 3.1.2.2.) in fresh and thawed sperm were determined. In comparison with the control group, egg fertilization with cryopreserved milt varied from 6.6% to 96.0%, depending on the donor male. Fertilization success with cryopreserved pooled milt was 71.8%. Freezing and thawing procedure caused loss of proteins from injured spermatozoa, resulting in significantly lower enzymatic activity in spermatozoa. Intensity of enzyme leakage in thawed milt correlated negatively with fertilization success. Concentration of spermatozoa could be a possible accessory quality indication, useful when selecting sperm samples appropriate for cryopreservation. |
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