DPC与DTA-6复配对转基因抗虫棉根系功能的调控

以双价转基因抗虫棉sGK321为供试品种,在田间种植条件下研究了DPC与DTA-6复配对抗虫棉根系功能的调控作用。结果表明,与单独应用DPC相比,DPC与DTA-6复配可以更有效地提高转基因抗虫棉的根系活力、增强根系合成氨基酸和细胞分裂素的能力、增强根系吸收和运输NO3-的能力。DPC及其与DTA-6的复配剂均降低了根系对Pi和K的吸收及运输,这可能与抗虫棉的Pi,K体内利用效率提高有关。经韧皮部运向根系的蔗糖增加是DPC与DTA-6复配后改善转基因抗虫棉根系功能的物质基础。

The Effects of Mixture of DPC and DTA-6 on Root Function of Transgenic Inect-Resistant Cotton

Transgenic insect-resistant cotton cultivars senesced prematurely more often than conventional cultivars in the North China Plain,which was likely related to its poor root function.We have found that mixture of DPC(mepiquat chloride) and DTA-6(diethyl am inoethyl hexanoate) could improve the root development of transgenic insect-resistant cotton at seedling stage.The objective of this research is to further investigate the effect of mixture of DPC and DTA-6 on root function of transgenic insect-resistant cotton cultivar sGK321 from square stage to mass boll-forming stage under field condition.Compared with single application of DPC,mixture of DPC and DTA-6 improved the root function of sGK321 more effectively.For example,it had stronger root activity,synthesized more amino acids and cytokinin,uptaked and transported more NO_3~-.However,DPC and mixture of DPC and DTA-6 decreased the uptake and transportation of Pi and K,which was possibly attributed to the higher use efficiency of them in plant.The increase of sugar allocated to root through phloem was responsible for the enhancement of root function of sGK321 by mixture of DPC and DTA-6.The results suggested that the application of mixture of DPC and DTA-6 could be used to prevent premature senescence of transgenic insect-resistant cotton.