Technology of In Vitro production of dihaploid Brassica plants

The conditions of cultivating isolated anthers and microspores are optimized for the purpose of producing haploid rapeseed, white head cabbage, and cauliflower plants under in vitro conditions and the process of direct embryogenesis is studied. It is established that the presence of 1 mg/l kinetin and 1 mg/l naphthaleneacetic acid hormones in nutrient medium B5 stimulated the process of direct embryogenesis in rapeseed as well as the formation of secondary and tertiary embryoids on hypocotyl and leaf segments of sterile sprouts. A cytological analysis of regenerant plants (count of the number of chloroplasts in stomatal guard cells and chromosomes in root meristem) confirmed the haploid nature of the rapeseed plants. Regenerants are obtained from callus tissue of cabbage microspores.