Poly(methyl methacrylate-glycidiyl methacrylate) film with immobilized iminodiacetic acid and Cu(II) Ion: For protein adsorption |
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Authors: | Gulay Bayramoglu M Yakup Arica |
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Institution: | 1. Biochemical Processing and Biomaterial Research Laboratory, Faculty of Sciences, Gazi University, 06500, Teknikokullar, Ankara, Turkey
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Abstract: | Poly(methyl methacrylate-glycidiyl methacrylate) p(MMA-GMA) film was fabricated by UV initiated photo-polymerization. Iminodiacetic acid ligand (IDA) was covalently immobilized to the p(MMA-GMA) film via ring opening reaction of epoxy groups. Cu(II) ion was chelated with the immobilized ligand. The morphology and properties of the films were characterized with SEM, FTIR, AFM, water content, the specific surface area, and contact angle. HSA (human serum albumin) was used as a model protein to describe the adsorption propency of the support. The information obtained in this research will serve for scaling up the process in industrial applications. The HSA adsorption capacity of the metal chelated film was found to be 2.7 mg/cm2 film. The p(MMA-GMA)-IDA-Cu(II) films exhibited a high adsorption capacity and fast adsorption rate compared to Cu(II) ion free counterpart. |
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