Sensitivity and specificity of various serological tests for the detection of Toxoplasma gondii infection in naturally infected sheep |
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Authors: | Shaapan R M El-Nawawi F A Tawfik M A A |
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Affiliation: | Department of Zoonosis, National Research Center, Giza, Egypt. rmshaapan2005@yahoo.com |
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Abstract: | Comparative serological examination of 300 serum samples from sheep slaughtered in the main abattoir in Cairo, Egypt revealed a higher prevalence of toxoplasmosis (43.7%) with the modified agglutination test (MAT), followed by the enzyme linked immune-sorbant assay (ELISA) (41.7%) and the indirect fluorescent antibody test (IFAT) (37%), while the lowest prevalence was detected with the dye test (DT) (34%). When the data from the first three serological tests were compared with that of the DT test, which was used as a reference test for toxoplasmosis, MAT had the highest sensitivity (96%), followed by ELISA (90.1%) and IFAT, which demonstrated the lowest sensitivity (80.4%). Conversely, IFAT had the highest specificity (91.4%), followed by MAT (88.9%) and ELISA (85.9%). |
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