水体理化因子对感染WSSV凡纳滨对虾存活时间的综合影响

对文献报道的单因子试验数据进行挖掘,收集盐度、NH_4~+-N和NO_2~--N、pH、WSSV注射量等5个因子对凡纳滨对虾(Litopenaeus vannamei)发病后存活时间影响的独立试验数据,重新整理后进行多因素方差分析和多元回归分析。结果显示,盐度、pH和WSSV注射量对凡纳滨对虾存活时间的影响极显著,NH_4~+-N和NO_2~--N的影响不明显,与文献报道的单因子试验结果一致。多元回归分析结果显示,pH和WSSV注射量与凡纳滨对虾存活时间的相关性极显著,回归方程为"Y(存活时间)=827.6-57.5 X_1(pH)-0.045 X_2(WSSV注射量)",R=0.820,其他3个因子的影响与凡纳滨对虾存活时间的线性关系均不明显。利用单因子数据综合分析的结果与文献报道的多因素试验结果一致,表明对文献数据进行综合分析的方法可行,为凡纳滨对虾疾病综合防控研究提供新的思路。

Effect of Water Physiochemical Factors on Survival Time of Litopenaeus vannamei Infected by WSSV

Data about the effect of 5 factors,such as salinity,NH⁺₄-N,NO^-₂-N,pH and WSSV copies,on survival time of L. vannamei had been collected from different published articles,and retreated normally for multivariate analysis of variance and multiplelinear regression analysis. Results showed that salinity,pH and WSSV copies injected affected the survival time of L. vannamei significantly,but effects of NH⁺₄-N and NO^-₂-N were not significant,coinciding with results of the published articles. Results of liner regression analysis showed that only effects of pH and WSSV copies on the survival time of L. vannamei were significant,and the regression equation was Y(survival time)=827.6-57.5X₁(pH)-0.045X₂(WSSV copies),with R=0.820. The liner effects of the other three factors on the survival time of L. vannamei were all not significant. In conclusion,the associated analysis for data from one factor experiments gets results identical with the results of multiple factors experiment,indicating that reusing and associated analysis of data from published articles is feasible,and would be a novel method for research on disease control of L. vannamei.