| 5-羟色胺对肉鸡肺动脉平滑肌细胞增殖和凋亡的影响 |
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| 引用本文： | 李英,曾健滢,韩前彪,唐兆新,郭剑英,潘家强.5-羟色胺对肉鸡肺动脉平滑肌细胞增殖和凋亡的影响[J].中国兽医学报,2011,31(8). |
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| 作者姓名： | 李英 曾健滢 韩前彪 唐兆新 郭剑英 潘家强 |
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| 作者单位： | 华南农业大学兽医学院,广东广州,510642 |
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| 基金项目： | 国家自然科学基金资助项目(30600440) |
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| 摘 要： | 采用胶原酶消化法培养原代肉鸡肺动脉平滑肌细胞（PASMC）,检测不同浓度的5-羟色胺（5-HT）对肉鸡PASMC增殖的影响,并用流式细胞仪检测PASMC周期和凋亡情况。结果发现,10-4～10-7 mol/L的5-HT能剂量依赖性地促进肉鸡PASMC的增殖,可能是5-HT加速细胞从G1期向S期过渡,同时5-HT也可促进PASMC凋亡,因此在总体上5-HT促进PASMC增殖的作用更明显。
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| 关 键 词： | 肺动脉平滑肌细胞 5-羟色胺 肉鸡 |
Effect of serotonin on the cell proliferation and apoptosis of cultured pulmonary arterial smooth cell of broiler |
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| LI Ying,ZENG Jian-yin,HAN Qian-biao,TANG Zhao-xin,GUO Jian-ying,PAN Jia-qiang.Effect of serotonin on the cell proliferation and apoptosis of cultured pulmonary arterial smooth cell of broiler[J].Chinese Journal of Veterinary Science,2011,31(8). |
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| Authors: | LI Ying ZENG Jian-yin HAN Qian-biao TANG Zhao-xin GUO Jian-ying PAN Jia-qiang |
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| Institution: | LI Ying,ZENG Jian-yin,HAN Qian-biao,TANG Zhao-xin,GUO Jian-ying,PAN Jia-qiang(College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong 510642,China) |
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| Abstract: | Primary pulmonary artery smooth muscle cells(PASMC) of broiler were cultured with the collagenase digestion method.Effect of different concentrations of serotonin(5-HT) on broiler PASMC proliferation was detected.Cell cycle and apoptosis of PASMC were detected by using flow cytometry.The results showed that 10-4-10-7mol/L of 5-HT can promote the proliferation of broiler PASMC dose-dependently,it is potential that 5-HT can accelerate the cell transition from G1 phase to S phase,while 5-HT could also contribu... |
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| Keywords: | pulmonary artery smooth muscle cells serotonin broiler |
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