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重庆地区家畜附红细胞体分子流行病学调查及危险性因素
引用本文:聂奎,周作勇,周荣琼,罗洪林,唐成,胡世君,汤明,胡友兰,於剑.重庆地区家畜附红细胞体分子流行病学调查及危险性因素[J].中国兽医学报,2011,31(6).
作者姓名:聂奎  周作勇  周荣琼  罗洪林  唐成  胡世君  汤明  胡友兰  於剑
作者单位:1. 西南大学,动物科技学院,重庆北碚400715
2. 西南大学荣昌校区动物医学系,重庆荣昌,402460
3. 重庆市农业局动物防疫检疫处,重庆,401147
4. 重庆市中药研究院,重庆,400065
基金项目:重庆市自然科学基金重点资助项目(CSTC,2006BA1009)
摘    要:本试验对重庆地区牛、猪和羊附红细胞体进行分子流行病学调查及危险性因素分析。在2006—2008年期间,采用鲜血压片法、姬姆萨染色法和基于16SrRNA-PCR方法,共检测了307头份牛血样,检测阳性率分别为46%、48%和11%;检测猪血样1 658头份,检测阳性率分别为63%、68%和76%;检测羊血样1 191份,检测阳性率分别为61%、70.4%和16.1%。危险性因素分析结果显示,山区牛附红细胞体感染阳性率极显著高于丘陵地区(OR=16.03,95%CI=5.47~17.11)(P<0.01),山区猪和山羊附红细胞体感染阳性率显著高于丘陵地区(猪:OR=1.38,95%CI=1.06~1.79;羊:OR=1.43,95%CI=0.54~3.82)(P<0.05)。农户散养牛和猪的附红细胞体感染阳性率极显著高于规模化养殖(牛:OR=3.2,95%CI=1.48~6.89;猪:OR=2.28,95%CI=1.79~2.89)(P<0.01)。农户散养山羊附红细胞体感染阳性率高于规模化养殖场(OR=1.19,95%CI=1.09~1.54)(P>0.05),但差异不显著。

关 键 词:      附红细胞体  流行病学  危险性因素  

Molecular epidemiological and risk factors of livestock Eperythrozoonosis in Chongqing
NIE Kui,ZHOU Zuo-yong,ZHOU Rong-qiong,LUO Hong-lin,TANG Cheng,HU Shi-jun,TANG Ming,HU You-lan,YU Jian.Molecular epidemiological and risk factors of livestock Eperythrozoonosis in Chongqing[J].Chinese Journal of Veterinary Science,2011,31(6).
Authors:NIE Kui  ZHOU Zuo-yong  ZHOU Rong-qiong  LUO Hong-lin  TANG Cheng  HU Shi-jun  TANG Ming  HU You-lan  YU Jian
Institution:NIE Kui1,ZHOU Zuo-yong2,ZHOU Rong-qiong2,LUO Hong-lin1,TANG Cheng1,HU Shi-jun2,TANG Ming3,HU You-lan3,YU Jian4(1.College of Animal Science and Technology,Southwest University,Beibei,Chongqing 400715,China,2.Rongchang,Campus,Rongchang,Chongqing 402460,3.Chongqing Animal Health Supervision Station,Chongqing 401147,4.Chongqing Academy of Chinese Materia Media,Chongqing 400065,China)
Abstract:In this study,we performed molecular epidemiological surveys and risk factors analysis of Eperythrozoonosis for cattle,pig and goat in Chongqing.Samples were collected from 2006 to 2008 and Eperythrozoon infection were detected by three different methods,including blood smear,Giemsa stain assay and 16S rRNA-PCR.The prevalence of Eperythrozoon infection was 46%,48% and 11% in cattle,61%,70.4% and 16.1% in goat,and 63%,68% and 76% in pig by blood smear,Giemsa stain assay and 16S rRNA-PCR methods,respectively....
Keywords:cattle  pig  goat  Eperythrozoon  molecular epidemiology  risk factors  
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