| 山羊痘、羊口疮及山羊传染性胸膜肺炎混合感染的检测与分析 |
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| 引用本文： | 颜新敏,储岳峰,吴国华,李健,朱海霞,朱彩珠,张强. 山羊痘、羊口疮及山羊传染性胸膜肺炎混合感染的检测与分析[J]. 中国兽医学报, 2011, 31(6) |
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| 作者姓名： | 颜新敏 储岳峰 吴国华 李健 朱海霞 朱彩珠 张强 |
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| 作者单位： | 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,农业部草食动物疫病重点开放实验室,甘肃兰州730046 |
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| 基金项目： | 甘肃省重大科技专项资助项目(092NKDA032); 国家科技支撑计划资助项目(2006BAD06A11,2006BAD06A17); 国家科技基础性工作专项计划资助项目(2008FY210200) |
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| 摘 要： | 利用特异性PCR方法从病山羊组织病料中分别扩增山羊痘病毒、羊口疮病毒和山羊支原体山羊肺炎亚种特异性片段,继而将扩增的特异性片段克隆、测序,并与GenBank上相应的基因序列进行比对、绘制系统进化树,进行流行病学分析。结果表明,从病山羊中可同时扩增出山羊痘病毒、羊口疮病毒和山羊支原体山羊肺炎亚种特异性片段,并通过基因序列比对分析确证了PCR检测结果,首次证实我国存在山羊痘、羊口疮及山羊传染性胸膜肺炎的混合感染,为我国现阶段羊病的流行病学和有效防制措施的制定提供了参考依据。
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| 关 键 词： | 山羊痘病毒 口疮病毒 羊支原体山羊肺炎亚种 PCR 混合感染 |
Detection and analysis of mixed infection of GPV,CPDV and Mccp in goats |
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| YAN Xin-min,CHU Yue-feng,WU Guo-hua,LI Jian,ZHU Hai-xia,ZHU Cai-zhu,ZHANG Qiang. Detection and analysis of mixed infection of GPV,CPDV and Mccp in goats[J]. Chinese Journal of Veterinary Science, 2011, 31(6) |
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| Authors: | YAN Xin-min CHU Yue-feng WU Guo-hua LI Jian ZHU Hai-xia ZHU Cai-zhu ZHANG Qiang |
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| Affiliation: | YAN Xin-min,CHU Yue-feng,WU Guo-hua,LI Jian,ZHU Hai-xia,ZHU Cai-zhu,ZHANG Qiang(Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture,Key Laboratory of Animal Virology of Ministry of Agriculture,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China) |
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| Abstract: | Specific gene fragments for goat pox virus(GPV),contagious pustular dermatitis virus(CPDV) and M.capricolum subsp.capripneumonia(Mccp) were amplified from the same goat in a herd located in Chongqing.Comparative sequences analysis of these specific genes from this case were carried out and their phylogenetic relationship with other related sequences available in GenBank were determined,respectively.The report firstly described the occurrence of mixed infection of GPV,CPDV and Mccp in caprine in China. |
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| Keywords: | GPV CPDV Mccp PCR co-infection |
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