| 猪源链球菌对大环内酯类主要耐药基因的检测 |
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| 引用本文： | 芮萍,马增军,陈翠珍,段玲欣,郭玉芳,房海.猪源链球菌对大环内酯类主要耐药基因的检测[J].中国兽医学报,2011,31(6). |
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| 作者姓名： | 芮萍 马增军 陈翠珍 段玲欣 郭玉芳 房海 |
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| 作者单位： | 河北科技师范学院河,北省预防兽医学重点实验室,河北秦皇岛066004 |
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| 基金项目： | 河北省科技厅科技支撑项目(09220402D); 河北省自然基金资助项目(C2009000877); 河北省教育厅计划项目(2008448); 河北省畜牧局计划项目(20070304) |
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| 摘 要： | 为了解猪源链球菌对红霉素相关耐药基因ermB、ermA和mefA的分布,对河北省及辽宁部分地区的64株猪源链球菌分离株,用PCR方法检测51株红霉素耐药株和13株红霉素敏感株中ermB、ermA和mefA基因。结果显示,耐药菌株中ermB基因的检出率为98.04%(50/51),ermA的检出率为25.49%(13/51),没有检出mefA基因。初步表明河北省及辽宁部分地区的猪源链球菌对红霉素的耐药机制以ermB基因介导为主。20株菌的ermB基因核苷酸序列与GenBank中同源序列相似性为99%～100%。与参照序列AJ972604.1相比,20株菌的ermB氨基酸序列的突变位点较少,主要有Thr 75→Ser、Ser 100→Asn、Arg 118→His、Leu 175→Ile,以100和118位突变为主,进一步说明ermB基因是相对稳定的。
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| 关 键 词： | 猪 链球菌 大环内酯类抗生素 耐药基因 |
Detection of the major macrolide resistance genes in pig Streptococcus isolates |
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| RUI Ping,MA Zeng-jun,CHEN Cui-zhen,DUAN Ling-xin,GUO Yu-fang,FANG Hai.Detection of the major macrolide resistance genes in pig Streptococcus isolates[J].Chinese Journal of Veterinary Science,2011,31(6). |
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| Authors: | RUI Ping MA Zeng-jun CHEN Cui-zhen DUAN Ling-xin GUO Yu-fang FANG Hai |
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| Institution: | RUI Ping,MA Zeng-jun,CHEN Cui-zhen,DUAN Ling-xin,GUO Yu-fang,FANG Hai(Key Laboratory of Perventive Veterinary Medicine of Hebei,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066004,China) |
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| Abstract: | To investigate the distribution of ermB,ermA and mefA that related to the erythromycin resistance gene,64 pig Streptococcus isolates from different areas in Hebei and Liaoning provinces were detected by PCR amplification.The results showed that,among the 51 drug-resistant strains,98.04%(50/51) harbored ermB gene,25.49%(13/51) isolates contained ermA gene,and none of these strains was positive for mefA gene.It is considered that erythromycin resistance mechanism of pig Streptococcus isolates is mainly mediat... |
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| Keywords: | pigs Streptococcus macrolides antibiotics drug-resistance genes |
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