| 1型和2型鸭疫里默氏菌的交叉保护 |
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| 引用本文： | 程龙飞,傅光华,彭春香,施少华,黄瑜,陈红梅,万春和.1型和2型鸭疫里默氏菌的交叉保护[J].福建农林大学学报(自然科学版),2009,38(5). |
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| 作者姓名： | 程龙飞 傅光华 彭春香 施少华 黄瑜 陈红梅 万春和 |
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| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所,福建,福州,350013;福建省畜禽疫病防治工程技术研究中心,福建,福州,350013
2. 福建省农业科学院畜牧兽医研究所,福建,福州,350013 |
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| 基金项目： | 福建省自然科学基金资助项目，福建省科技厅重大专项资助项目，福建省科技厅科技计划项目，福建省财政厅专项资助项目 |
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| 摘 要： | 通过PCR扩增、克隆、序列测定获得29株鸭疫里默氏菌(RA)的16S rRNA和16S-23S rRNA间隔区的核苷酸序列.将16S rRNA和16S-23S rRNA间隔区序列连接后分析发现,1型菌株和大多数2型菌株分别处于不同的分支,而2型菌株中的RAf11、RAf3和RAf104不处在2型菌株的分支中,却与1型菌株非常接近.挑选RAf11菌株和2型分支中的RAf19菌株,分别制备灭活苗,免疫雏鸭后进行攻菌保护试验,RAf11菌株对1型菌株的攻击可产生较高的交叉保护率,达53.8%,而RAf19菌株对1型菌株攻击的保护率仅为23.1%.借助16S rRNA和16S-23S rRNA间隔区序列的分析,筛选到一株具交叉保护的RA菌株.
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| 关 键 词： | 鸭疫里默氏菌 16S rRNA序列 16S-23S rRNA间隔区序列 交叉保护 |
The cross-protection between Riemerella anatipestifer serotype 1 and 2 |
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| CHENG Long-fei,FU Guan-hua,PENG Chun-xiang,SHI Shao-hua,HUANG Yu,CHEN Hong-mei,WAN Chun-he.The cross-protection between Riemerella anatipestifer serotype 1 and 2[J].Journal of Fujian Agricultural and Forestry University,2009,38(5). |
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| Authors: | CHENG Long-fei FU Guan-hua PENG Chun-xiang SHI Shao-hua HUANG Yu CHEN Hong-mei WAN Chun-he |
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| Institution: | CHENG Long-fei1,2,FU Guan-hua1,PENG Chun-xiang1,SHI Shaohua1,HUANG Yu1,CHEN Hong-mei1,WAN Chun-he1,2(1.Animal Husbandry and Veterinary Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China,2.Fujian Animal Diseases Control Technology Development Center,China) |
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| Abstract: | The sequences of 16S rDNA and 16S-23S rDNA intergenic spacers of 29 strains of Riemerella anatipestifer(RA) were determined.Phylogenetic analysis indicated that the serotype 1 strains and the majority of serotype 2 strains formed different branches,whereas serotype 2 strains RAf11,RAf3 and RAf104 were found to be closely related to serotype 1.Inactivated vaccines of RA serotype 2 were prepared using strain RAf19 in serotype 2 branch and strain RAf11 in serotype 1 branch,respectively.After immunization,the d... |
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| Keywords: | Riemerella anatipestifer 16S rRNA genes 16S-23S rRNA intergenic spacer sequence cross-protection |
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