30味中药提取物与环丙沙星联用对猪源链球菌体外抑菌作用研究

为筛选与环丙沙星联用对猪源链球菌具有协同抑菌作用的中药提取物，采用试管二倍稀释法测定30味中药醇提物、水提物的最低抑菌浓度（MIC），采用棋盘稀释法测定30味中药醇提物、水提物与环丙沙星联用对猪源链球菌的部分抑菌浓度（FIC），采用有机溶剂萃取法初步追踪与环丙沙星联用具有协同抑菌作用的中药萃取物的体外抑菌效果。结果显示，所选30味中药的水提物、醇提物对猪源链球菌均具有一定的抑菌作用，佩兰、黄连、枳壳、郁金等中药醇提物、水提物对猪源链球菌（pf—ce）抑菌活性较强，苍术水提物等对猪源链球菌的抑菌作用较弱；佩兰醇提物与环丙沙星联用对猪源链球菌的协同抑菌作用最强；佩兰醇提物的乙酸乙酯萃取物、氯仿萃取物与环丙沙星联用对猪源链球菌分别具有协同、相加抑菌作用。

Inhibitory effect of combination of thirty Chi-nese herbs extract with Ciprofloxacin against Streptococcus suis in vitro

To screen synergistic inhibitory effect of active ingredients from Chinese herbs combined with ciprofloxacin against Streptococcus suis in vitro. Minimum inhibitory concentration （MIC） of water exact and alcohol extract from thirty Chinese herbs against streptococcus suis in vitro were determined by two-double dilution method. Fractional inhibitory concentrations （FIC） of water exact and alcohol exact from thirty Chinese herbs combined with antibiotics against Streptococcus suis were determined by constant checkerboard dilution method. To track inhibitory effect from Chinese herds with synergistic inhibitory effect combine with ciprofloxacin against Streptococcus suis in vitro by organic extraction method. The results showed that water extract and alcohol extract from thirty medicine herbs have antibactierial effect against streptococcus suis in certain extent. It is showen that some extracts such as alcohol extract , water extract of Eupatorium fortunei Turcz, Coptidis Rhizoma, Aurantii Fructus, Curcumae Radix have stronger antibactierial activity against on Streptococcus suis in vitro. And the antibactierial activity of some extracts such as water extract of Atractylodis Rhizoma have weaker inhibitory effect on streptococcus suis in vitro. Inhibitory bacterium additive effect was existed in the alcohol extract of Eupatorium fortunei Turcz combine with ciprofloxaxin. Inhibitory bacterium additive effect, synergistic inhibitory effect was existed in some extracts such as chloroform extract and ethyl acetate extract of alcohol extract from Eupatorium fortune Turcz combined with ciprofloxacin on streptococcus suis in vitro.