Effects of benomyl,carbendazim and thiophanates on respiration and oxidative phosphorylation of Fusarium oxysporum and Saccharomyces cerevisiae

Benomyl methyl 1-(butylcarbamoyl)-benzimidazol-2-ylcarbamate] (350 × 10^?6 M) decreased the respiration rate of Fusarium oxysporum conidia by 50% during germination. This inhibition was maintained at least 24 h after the treatment had begun. The treatment did not modify the relation between incubation time and respiration rate. Carbendazim methyl benzimidazol-2-ylcarbamate], thiabendazole2-(thiazol-4-yl)benzimidazole], thiophanate 1,2-di-(3-ethoxycarbonyl-2-thioureido)benzene] and thiophanate-methyl 1,2-di-(3-methoxycarbonyl-2-thioureido)benzene] were assayed using isolated mitochondria of Saccharomyces cerevisiae. These four compounds decreased mitochondrial respiration and oxidative phosphorylation rates to different extents when they were applied at a concentration of 250 × 10^?6 M . Thiophanate-methyl was the most effective since it completely suppressed the mitochondrial respiratory control at 75 × 10^?6 M .