|
|||||
|
|
| 毛竹种子“以芽繁芽”组培快繁初步研究 | |
| 引用本文： | 李蓉,郭起荣,曾炳山,何高峰. 毛竹种子“以芽繁芽”组培快繁初步研究[J]. 世界竹藤通讯, 2008, 6(6): 9-13 |
| 作者姓名： | 李蓉 郭起荣 曾炳山 何高峰 |
| 作者单位： | 1. 广州市林业科学研究所有限公司,广东,510515 2. 国际竹藤网络中心,北京,100102 3. 中国林业科学研究院热带林业研究所,广州,510520 4. 江西南昌市林业局,南昌,330038 |
| 基金项目： | 国家自然基金30460111;科技支撑2006BAD19B0305资助科技条件;平台项目2005DKA21003 |
| 摘 要： | 以毛竹种子为外植体，通过“以芽繁芽”方式找到了较好的外植体消毒技术、增殖培养基、生根培养基和炼苗移栽技术等，初步建立了毛竹种胚外植体“以芽繁芽”组培快繁体系。其中最佳消毒时间为10％次氯酸钠溶液0．1％升汞各20min，消毒成功率可达37％；最佳增殖培养基为MS＋TDZ0．5mg／L＋KT2．0mg／L，增殖4-5代则衰退；生根培养基以1／2MS＋IBA1．0mg／L＋KT0．5mg／L＋AC0．1g／L效果较好；炼苗移植成活率可达69％，可应用生产。 |
| 关 键 词： | 毛竹 种子 以芽繁芽 增殖 生根 初探 |
A Preliminary Study of Tissue Culture and Propagation of Moso Bamboo by Shoot Proliferation |
|
| Li Rong,Guo Qirong,Zeng Bingshan,He Gaofeng. A Preliminary Study of Tissue Culture and Propagation of Moso Bamboo by Shoot Proliferation[J]. World Bamboo and Rattan, 2008, 6(6): 9-13 | |
| Authors: | Li Rong Guo Qirong Zeng Bingshan He Gaofeng |
| Affiliation: | 1Guangzhou Forestry Science Institute;Guangzhou;Guangdong;510515 2International Center for Bamboo and Rattan;Beijing;China;100102 3The Research Institute of Tropical Forestry;510520 4Nanchang Forestry Bureau;Nanchang;Jiangxi;330038 |
| Abstract: | This study optimized the technologies for disinfection of explants, propagation culture medium, rooting medium, seedlings transplanting, etc. by means of shoot proliferation with Moso bamboo seeds as explants, and preliminarily established the tissue culture and propagation system for explants of moso seeds by shoot proliferation. The best disinfection method is to immerse seed explants in 10% sodium hypochlorite for 20 minutes and then in 0.1% mercuric chloride for another 20 minutes, with the rate of disi... |
| Keywords: | Phyllostachys edulis seed shoot proliferation multiplication rooting |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |