Multiplex PCR-based identification of Streptococcus canis,Streptococcus zooepidemicus and Streptococcus dysgalactiae subspecies from dogs |
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Affiliation: | 1. Institute of Chemical Technology, Department of Biochemistry and Microbiology, Technicka 5, 166 28 Prague 6, Czech Republic;2. Institut Pasteur, Unité Prévention et Thérapie Moléculaires des Maladies Humaines, 25 rue du Dr Roux, 75015 Paris, France;3. Centre National de la Recherche Scientifique (CNRS) -URA 3012, Paris, France;1. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, Queensland 4067, Australia;2. Pig Services Centre, Agriculture, Food and Fibre Division, Department of Jobs, Precincts and Regions, Epsom, Victoria 3551, Australia;3. Department of Agriculture and Fisheries, Ecosciences Precinct, Dutton Park, Queensland 4102, Australia;4. Apiam Animal Health, Bendigo, Victoria 3550, Australia |
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Abstract: | Streptococcus canis (S. canis), Streptococcus equi subspecies zooepidemicus (S. zooepidemicus) and Streptococcus dysgalactiae subspecies (S. dysgalactiae subspecies) are β-haemolytic Gram positive bacteria infecting animals and humans. S. canis and S. zooepidemicus are considered as two of the major zoonotic species of Streptococcus, while more research is needed on S. dysgalactiae subspecies bacteria. In this work, a multiplex-PCR protocol was tested on strains and clinical samples to detect S. canis, S. dysgalactiae subspecies and S. equi subspecies bacteria in dogs. All strains were correctly identified as S. canis, S. equi subspecies or S. dysgalactiae subspecies by the multiplex-PCR. The main Streptococcus species isolated from symptomatic dogs were confirmed S. canis. The multiplex-PCR protocol described is a rapid, accurate and efficient method for identifying S. canis, S. equi subspecies and S. dysgalactiae subspecies in dogs and could be used for diagnostic purposes and for epidemiological studies. |
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Keywords: | Dogs Humans Multiplex-PCR Zoonosis |
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