Genetic diversity and antibiotic susceptibility of Staphylococcus aureus isolates from wild boars |
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| Institution: | 1. Institute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany;2. Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Services, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany;2. Institute of Agricultural Sciences (IAS), Animal Physiology Group, ETH Zurich, 8092 Zurich, Switzerland;3. Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland;1. Division of Respiratory and Critical Care Medicine, Department of Internal Medicine, Taichung Hospital, Ministry of Health and Welfare, Taichung, Taiwan;2. Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan;3. Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan;4. Division of Respiratory and Critical Care Medicine, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan;5. Department of Laboratory Medicine, Linkou Chang-Gung Memorial Hospital, Taoyuan, Taiwan;6. Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Kweishan, Taoyuan, Taiwan;2. Department of Food Hygiene and Food Technology, Veterinary Faculty, University of León, E-24071, León, Spain;3. Institute of Food Science and Technology, University of León, E-24007, León, Spain;4. Department of Veterinary Science, University of Trás-os-Montes and Alto Douro, 5001-911, Vila Real, Portugal;5. Centre for Animal and Veterinary Science, University of Trás-os-Montes and Alto Douro, 5001-911, Vila Real, Portugal;6. Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-911, Vila Real, Portugal;7. Institute for Biotechnology and Bioengineering, Centre for Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-911, Vila Real, Portugal;1. Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, Merelbeke, Belgium;2. Department of Veterinary Medicine and Animal Production, University of Naples “Federico II”, Via Delpino 1, 80137 Naples, Italy;3. Task Force on Microbiome Studies, University of Naples “Federico II”, Naples, Italy;4. Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, Karel Lodewijk Ledeganckstraat 35, 9000 Ghent, Belgium |
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| Abstract: | We here report the occurrence of S. aureus in wild boars and characterize isolates genotypically and phenotypically in order to get knowledge about the occurrence of clonal lineages and genotypes in free-living wild animals. Forty-one S. aureus isolates obtained from 111 wild boars hunted in Lower Saxony, Germany, were investigated and compared to human and livestock isolates. The S. aureus belonged to multilocus sequence types ST1, ST7, ST30, ST133, ST425, ST804, ST890 and to the new ST3237, ST3238, ST3255 and ST3369. The livestock associated CC398-MRSA lineage, however, was not found. In addition to well-known spa types, the new types t14999, t15000, t15001 and t15002 were detected. Macrorestriction analysis revealed a variety of different SmaI fragment patterns. Most isolates were susceptible to all antimicrobials tested, including methicillin, and resistance was detected only to ampicillin, penicillin and erythromycin. PCR analysis confirmed the presence of staphylococcal enterotoxin genes (seh) in all t127-ST1 isolates. A high degree of genetic diversity was detected with many spa types and clonal lineages previously reported in humans and livestock animals. |
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| Keywords: | Wild boars Antimicrobial susceptibility MLST |
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