Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution |
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| Institution: | 1. Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral), Universidad Nacional del Litoral (UNL)/Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina;2. Departamento de Ciencias Morfológicas, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral (UNL), Esperanza, Santa Fe, Argentina;3. Departamento de Clínicas, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral (UNL), Esperanza, Santa Fe, Argentina;4. Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria (INTA), Rafaela, Santa Fe, Argentina;1. Department of Orthopedics, the Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China;2. Department of Hand Surgery, Huashan Hospital and Institutes of Biomedical Sciences, Fudan University, Shanghai, China;3. Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai, China;1. Département de biologie, Faculté des sciences, Université de Sherbrooke, 2500 boul. de l’Université, Sherbrooke, QC, J1K 2R1, Canada;2. Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada, 2000 College, Sherbrooke, QC, J1M 0C8, Canada;1. Department and Clinic of Reproduction, University of Life Sciences in Lublin, G??boka, Lublin, Poland;2. Department of Epizootiology and Clinic of Infectious Diseases, University of Life Sciences in Lublin, G??boka, Lublin, Poland;3. Department and Clinic of Animal Surgery, University of Life Sciences in Lublin, G??boka, Lublin, Poland;4. Department and Clinic of Internal Medicine, University of Life Sciences in Lublin, G??boka, Lublin, Poland;1. MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;2. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China;3. Department of Livestock and Fisheries, Ministry of Agriculture and Forestry, Vientiane, Laos;1. Dairy Hygiene Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, 4 Hitsujigaoka, Toyohira, Sapporo, Hokkaido, 062-0045, Japan;2. Division of Health and Science, School of Veterinary Medicine, Rakuno Gakuen University, 582, Bunkyodai-Midorimachi, Ebetsu, Hokkaido, 069-8501, Japan;3. Hokkaido Tokachi Livestock Hygiene Service Center, 59-6 Kisen, Kawanishicho, Obihiro, Hokkaido, 089-1182, Japan;4. Hokkaido Chuou Agriculture and Mutual Aid Association Nayoro Large Animal Clinic, 136-47 Houei, Nayoro, Hokkaido, 096-0072, Japan |
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| Abstract: | The aim of this study was to characterize the immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution. Twenty-one Holstein non-pregnant cows in late lactation either uninfected or with chronic naturally acquired S. aureus intramammary infections (IMI) were included in this study. Cows were slaughtered at 7, 14 and 21 d after cessation of milking and samples for immunohistochemical analysis were taken. Protein expression of toll-like receptor 2 (TLR2) and TLR4 was significantly higher in S. aureus-infected quarters than in uninfected controls at the three involution stages studied. Protein expression of tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1α and IL-17 was significantly affected by IMI; being higher in S. aureus-infected than uninfected quarters during all evaluated stages. In S. aureus-infected and uninfected quarters protein expression of lactoferrin increased from day 7–14 of involution, decreasing significantly to day 21 in mammary quarters with chronic infections. The number of monocytes-macrophages was significantly higher in S. aureus-infected than in uninfected control quarters at 7 and 21 d of involution. The number of T lymphocytes was significantly higher in S. aureus-infected than in uninfected quarters at 7 and 14 d of involution while the number of B lymphocytes was significantly higher in S. aureus-infected than in uninfected quarters during all evaluated stages, showing a progressive increase as involution advanced. These results demonstrated a sustained and exacerbated innate and adaptive immune response during chronic S. aureus IMI, playing a critical role in the infection control during active involution. |
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| Keywords: | Immune response Mastitis Involution |
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