Immunohistochemical expression of interferon-γ in different types of granulomatous lesions associated with bovine paratuberculosis |
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| Institution: | 1. Dpto de Sanidad Animal, Instituto de Ganadería de Montaña (CSIC-Universidad de León), Facultad de Veterinaria, Campus de Vegazana s/n, 24071, León, Spain;2. Dpto de Sanidad Animal, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, 48160 Derio, Bizkaia, Spain;2. National Milk Records plc, Chippenham, SN15 1BN, United Kingdom;1. University of Guelph, Department of Population Medicine, Guelph, ON, Canada;2. Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, 160 Research Lane, Suite 206, Guelph, Ontario N1G 5B2, Canada;3. Food Safety and Quality, Agriculture and Consumer Protection Department, Food and Agriculture Organization, Viale delle Terme di Caracalla, Rome, Italy;4. Royal Veterinary College, North Mymms, Hertfordshire, UK;1. Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Molecular Pathogenesis, 07743 Jena, Naumburger Str. 96a, Germany;2. Friedrich Schiller University Jena, Faculty of Mathematics and Computer Science, RNA Bioinformatics and High Throughput Analysis, 07743 Jena, Leutragraben 1, Germany;2. National Milk Records plc, Chippenham, SN15 1BN, United Kingdom |
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| Abstract: | Animals infected with Mycobacterium avium subsp. paratuberculosis (Map) show a variety of lesions, from focal forms, seen in subclinical stages to diffuse lesions in clinical cases. The purpose of this study was to evaluate the local expression of IFN-γ by immunohistochemistry in relation with the type of lesion in naturally Map-infected cows. The number of immunolabelled cells, ?the majority morphologically consistent with lymphocytes-, was higher in focal and diffuse paucibacillary forms than in diffuse multibacillary lesions, where they appeared closely related to epithelioid cells. Diffuse multibacillary lesions had the lowest numbers, but higher than controls, and positive cells were intermingled among the macrophages. The peripheral IFN-γ production was higher in all Map infected cows and a positive correlation was found with the number of immunolabelled cells in the intestine. The findings of this study show that IFN-γ would play a role in the development of the different types of lesions in paratuberculosis, and also points out the importance of adequate sampling of lymphoid tissue containing samples when studying the local immune response in which IFN-γ expression may be involved, especially in cases where focal lesions are present. |
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| Keywords: | Paratuberculosis Bovine Immunohistochemistry IFN-γ Lesion |
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